Gallbladder microbial species and host bile acids biosynthesis linked to cholesterol gallstone comparing to pigment individuals

作者全名："Zhang, Xinpeng; Hu, Junqing; Li, Yi; Tang, Jichao; Yang, Kaijin; Zhong, Ayan; Liu, Yanjun; Zhang, Tongtong"

作者地址："[Zhang, Xinpeng; Li, Yi; Tang, Jichao; Yang, Kaijin; Zhong, Ayan] Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Gen Surg Day Ward, Dept Gen Surg,Affiliated Hosp, Chengdu, Peoples R China; [Zhang, Xinpeng; Hu, Junqing; Li, Yi; Tang, Jichao; Yang, Kaijin; Zhong, Ayan; Liu, Yanjun; Zhang, Tongtong] Chongqing Med Univ, Affiliated Hosp Chengdu 2, Chengdu, Peoples R China; [Hu, Junqing; Liu, Yanjun; Zhang, Tongtong] Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Ctr Gastrointestinal & Minimally Invas Surg, Dept Gen Surg,Affiliated Hosp, Chengdu, Peoples R China; [Hu, Junqing; Liu, Yanjun; Zhang, Tongtong] Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Ctr Obes & Metab Dis, Dept Gen Surg,Affiliated Hosp, Chengdu, Peoples R China; [Hu, Junqing; Zhang, Tongtong] Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Med Res Ctr, Affiliated Hosp, Chengdu, Peoples R China"

通信作者："Zhang, TT (通讯作者)，Chongqing Med Univ, Affiliated Hosp Chengdu 2, Chengdu, Peoples R China.; Zhang, TT (通讯作者)，Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Ctr Gastrointestinal & Minimally Invas Surg, Dept Gen Surg,Affiliated Hosp, Chengdu, Peoples R China.; Zhang, TT (通讯作者)，Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Ctr Obes & Metab Dis, Dept Gen Surg,Affiliated Hosp, Chengdu, Peoples R China.; Zhang, TT (通讯作者)，Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Med Res Ctr, Affiliated Hosp, Chengdu, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001190798600001

JCR分区：Q1

影响因子：4.6

年份：2024

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：cholesterol gallstone; gallbladder microbiota; 2bRAD-M; bile acids metabolism; LC-MS metabolomics; multiomics

摘要："Gallstones are crystalline deposits in the gallbladder that are traditionally classified as cholesterol, pigment, or mixed stones based on their composition. Microbiota and host metabolism variances among the different types of gallstones remain largely unclear. Here, the bile and gallstone microbial species spectra of 29 subjects with gallstone disease (GSD, 24 cholesterol and 5 pigment) were revealed by type IIB restriction site-associated DNA microbiome sequencing (2bRAD-M). Among them (21 subjects: 18 cholesterol and 3 pigment), plasma samples were subjected to liquid chromatography-mass spectrometry (LC-MS) untargeted metabolomics. The microbiome yielded 896 species comprising 882 bacteria, 13 fungi, and 1 archaeon. Microbial profiling revealed significant enrichment of Cutibacterium acnes and Microbacterium sp005774735 in gallstone and Agrobacterium pusense and Enterovirga sp013044135 in the bile of cholesterol GSD subjects. The metabolome revealed 2296 metabolites, in which malvidin 3-(6''-malonylglucoside), 2-Methylpropyl glucosinolate, and ergothioneine were markedly enriched in cholesterol GSD subjects. Metabolite set enrichment analysis (MSEA) demonstrated enriched bile acids biosynthesis in individuals with cholesterol GSD. Overall, the multi-omics analysis revealed that microbiota and host metabolism interaction perturbations differ depending on the disease type. Perturbed gallstone type-related microbiota may contribute to unbalanced bile acids metabolism in the gallbladder and host, representing a potential early diagnostic marker and therapeutic target for GSD."

基金机构："Chengdu High-level Key Clinical Specialty Construction Project; Science & Technology Department of Sichuan Province [2021YFS0101, 23NSFSC0739]"

基金资助正文："The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study is funded by the Chengdu High-level Key Clinical Specialty Construction Project, and Science & Technology Department of Sichuan Province (23NSFSC0739 and 2021YFS0101)."