Sirt1 Inhibits Atrial Fibrosis by Downregulating the Expression of the Transforming Growth Factor-f31/Smad Pathway

作者全名："Chen, Yiqi; Zhao, Shuting; Xiao, Hua"

作者地址："[Chen, Yiqi; Zhao, Shuting; Xiao, Hua] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Xiao, Hua] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing, Peoples R China"

通信作者："Xiao, H (通讯作者)，Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing, Peoples R China."

来源：ACTA CARDIOLOGICA SINICA

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001193293800009

JCR分区：Q3

影响因子：1.8

年份：2024

卷号：40

期号：2

开始页：225

结束页：234

文献类型：Article

关键词：Atrial fibrillation; Atrial fibroblasts; Atrial fibrosis; Sirt1; TGF-f1 pathway

摘要："Background: Atrial fibrosis is an important factor leading to atrial fibrillation, and the transforming growth factorf1/Smad pathway is a key factor in inducing atrial fibrosis. Sirt1 is a member of the histone deacetylase (sirtuin) family, and recent studies have proven its cardioprotective effects. Objectives: This study explored the effect of Sirt1 on atrial fibrosis through the transforming growth factor-f1/ Methods: We analyzed human right atrial appendage tissues and explored the relationship between Sirt1 and atrial fibrosis at the morphological, functional and molecular levels by Masson trichrome staining, immunofluorescence, real-time quantitative polymerase chain reaction and Western blot analysis. Rat atrial fibroblasts were extracted and treated by the Sirt1 agonist resveratrol, inhibitor sirtinol, and recombinant human transforming growth factorf1 protein. The expression levels of related proteins were detected by Western blot, and the effect on the migration of atrial fibroblasts was detected by wound healing assay. Results: We found that the expression of Sirt1 was reduced in the right atrial appendage tissues of patients with atrial fibrillation, and the degree of fibrosis was increased. In atrial fibroblasts, the activation of Sirt1 could inhibit the expression of transforming growth factor-f1/Smad and reduce the development of fibrosis, while inhibiting Sirt1 reduced its inhibitory effect on the transforming growth factor-f1/Smad pathway. Conclusions: These findings indicate that Sirt1 inhibits atrial fibrosis by downregulating the expression of the transforming growth factor-f1/Smad pathway, and provide potential targets for the treatment of atrial fibrillation."

基金机构："Natural Science Foundation of Chongqing, China [cstc2019jcyj-msx mX0857]"

基金资助正文："This study was supported by the Natural Science Foundation of Chongqing, China (No. cstc2019jcyj-msx mX0857) ."