Construction of a three-dimensional inflammation model of human bronchial epithelial cells BEAS-2B by using the self-assembling D-form peptide Sciobio-III

作者全名："Su, Di; Luo, Xinyi; Chen, Jialei; Lu, Na; Zhao, Jiawei; Wan, Yuan; Gao, Yu; Liu, Qichen; Luo, Zhongli"

作者地址："[Su, Di; Luo, Xinyi; Chen, Jialei; Lu, Na; Zhao, Jiawei; Wan, Yuan; Gao, Yu; Liu, Qichen; Luo, Zhongli] Chongqing Med Univ, Coll Basic Med Sci Mol Med, Chongqing 400016, Peoples R China; [Su, Di; Luo, Xinyi; Chen, Jialei; Lu, Na; Zhao, Jiawei; Wan, Yuan; Gao, Yu; Liu, Qichen; Luo, Zhongli] Chongqing Med Univ, Canc Res Ctr, Chongqing 400016, Peoples R China; [Luo, Xinyi] Univ Calif Irvine, Dept Mat Sci & Engn, Irvine, CA 92697 USA; [Wan, Yuan] Univ Iowa, Coll Engn, Roy J Carver Dept Biomed Engn, Iowa, IA 52242 USA"

通信作者："Luo, ZL (通讯作者)，Chongqing Med Univ, Coll Basic Med Sci Mol Med, Chongqing 400016, Peoples R China.; Luo, ZL (通讯作者)，Chongqing Med Univ, Canc Res Ctr, Chongqing 400016, Peoples R China."

来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001193983000001

JCR分区：Q3

影响因子：2.5

年份：2024

卷号：704

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Self-assembling D-form peptide; Self-assembly; 3D cell culture; 3D inflammation model

摘要："Human bronchial epithelial cells in the airway system, as the primary barrier between humans and the surrounding environment, assume a crucial function in orchestrating the processes of airway inflammation. Target to develop a new three-dimensional (3D) inflammatory model to airway system, and here we report a strategy by using self-assembling D-form peptide to cover the process. By testing physicochemical properties and biocompatibility of Sciobio-III, we confirmed that it can rapidly self-assembles under the trigger of ions to form a 3D nanonetwork-like scaffold, which supports 3D cell culture including the cell strains like BEAS-2B cells. Subsequently, inflammation model was established by lipopolysaccharide (LPS), the expression of some markers of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and interleukin-8 (IL-8), the levels of relevant inflammatory factors were measured by RT-qPCR and the secretion profile of inflammatory cytokines by ELISA, are obtained the quite difference effects in 2D and 3D microenvironment, which suggested Sciobio-III hydrogel is an ideal scaffold that create the microenvironment for 3D cell culture. Here we are success to establish a 3D inflammation model for airway system. This innovative model allows for rapid and accurate evaluation of drug metabolism and toxicological side effects, hope to use in drug screening for airway inflammatory diseases and beyond."

基金机构："Chengdu Sciobio Surgery Institute, China [CSSI-JZ20211021-0002]; National Natural Science Foundation of China [NSFC 31771101]"

基金资助正文："This work was supported by Chengdu Sciobio Surgery Institute, China (Project Number: CSSI-JZ20211021-0002) , Zhongli Luo was supported by the grant from the National Natural Science Foundation of China (Project Number: NSFC 31771101) ."