Study on the optimal time limit of frozen embryo transfer and the effect of a long-term frozen embryo on pregnancy outcome

作者全名："Wang, Xue-Jiao; Chen, Ming-Xing; Ruan, Ling-Ling; Tan, Li; Geng, Li-Hong; Yang, Hui-Jun; Fu, Li-Juan; Zhong, Zhao-Hui; Lv, Xing-Yu; Ding, Yu-Bin; Wan, Qi"

作者地址："[Wang, Xue-Jiao; Tan, Li; Geng, Li-Hong; Lv, Xing-Yu] Sichuan Jinxin Xinan Women & Children Hosp, Chengdu, Peoples R China; [Chen, Ming-Xing; Ruan, Ling-Ling; Ding, Yu-Bin] Chongqing Med Univ, Dept Obstet & Gynecol, Women & Childrens Hosp, Chongqing, Peoples R China; [Chen, Ming-Xing; Ruan, Ling-Ling; Fu, Li-Juan; Zhong, Zhao-Hui; Ding, Yu-Bin] Chongqing Med Univ, Sch Publ Hlth, Joint Int Res Lab Reprod & Dev, Minist Educ China, Chongqing, Peoples R China; [Yang, Hui-Jun] Qingdao Univ, Assisted Reprod Ctr, Shandong Prov Maternal & Child Hlth Care Hosp, Jinan, Peoples R China; [Fu, Li-Juan] Changsha Med Univ, Dept Pharmacol, Academician Workstat, Changsha, Peoples R China; [Wan, Qi] Sichuan Univ, West China Univ Second Hosp, Dept Obstet & Gynecol, Chengdu, Peoples R China; [Wan, Qi] Sichuan Univ, Minist Educ, Key Lab Birth Defects & Related Dis Women & Childr, Chengdu, Peoples R China; [Wan, Qi] Sichuan Univ, 20,Sect 3,Renmin South Rd, Chengdu 610041, Sichuan Provinc, Peoples R China"

通信作者："Wan, Q (通讯作者)，Sichuan Univ, 20,Sect 3,Renmin South Rd, Chengdu 610041, Sichuan Provinc, Peoples R China."

来源：MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001195749400049

JCR分区：Q2

影响因子：1.3

年份：2024

卷号：103

期号：13

开始页：　

结束页：　

文献类型：Article

关键词：Embryo cryopreservation; embryo cryopreservation duration; frozen embryo transfer; pregnancy outcomes

摘要："In this retrospective study conducted at Sichuan Jinxin Xinan Women and Children's Hospital spanning January 2015 to December 2021, our objective was to investigate the impact of embryo cryopreservation duration on outcomes in frozen embryo transfer. Participants, totaling 47,006 cycles, were classified into 3 groups based on cryopreservation duration: <= 1 year (Group 1), 1 to 6 years (Group 2), and >= 6 years (Group 3). Employing various statistical analyses, including 1-way ANOVA, Kruskal-Wallis test, chi-square test, and a generalized estimating equation model, we rigorously adjusted for confounding factors. Primary outcomes encompassed clinical pregnancy rate and Live Birth Rate (LBR), while secondary outcomes included biochemical pregnancy rate, multiple pregnancy rate, ectopic pregnancy rate, early and late miscarriage rates, preterm birth rate, neonatal birth weight, weeks at birth, and newborn sex. Patient distribution across cryopreservation duration groups was as follows: Group 1 (40,461 cycles), Group 2 (6337 cycles), and Group 3 (208 cycles). Postcontrolling for confounding factors, Group 1 exhibited a decreased likelihood of achieving biochemical pregnancy rate, clinical pregnancy rate, and LBR (OR < 1, aOR < 1, P < .05). Furthermore, an elevated incidence of ectopic pregnancy was observed (OR > 1, aOR > 1), notably significant after 6 years of freezing time [aOR = 4.141, 95% confidence intervals (1.013-16.921), P = .05]. Cryopreservation exceeding 1 year was associated with an increased risk of early miscarriage and preterm birth (OR > 1, aOR > 1). No statistically significant differences were observed in birth weight or sex between groups. However, male infant birth rates were consistently higher than those of female infants across all groups. In conclusion, favorable pregnancy outcomes align with embryo cryopreservation durations within 1 year, while freezing for more than 1 year may diminish clinical pregnancy and LBRs, concurrently elevating the risk of ectopic pregnancy and preterm birth."

基金机构：Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJZD-K202200408]; Medical Science and Technology Project of the Sichuan Provincial Health Committee [21PJ166]

基金资助正文：This study was funded by the Scientific and Technological Research Program of Chongqing Municipal Education Commission (Grant No. KJZD-K202200408) and supported by the Medical Science and Technology Project of the Sichuan Provincial Health Committee (Grant No. 21PJ166).