Study on the effect and mechanism of <i>Lacticaseibacillus rhamnosus</i> AFY06 on inflammation-associated colorectal cancer induced by AOM/DSS in mice

作者全名："Zhang, Jing; Zhang, Piyun; Li, Sijia; Yu, Ting; Lai, Xiangyu; He, Yongpeng"

作者地址："[Zhang, Jing; Li, Sijia] Chongqing Chem Ind Vocat Coll, Environm & Qual Inspect Coll, Chongqing, Peoples R China; [Zhang, Piyun] Chongqing Univ, Cent Hosp, Chongqing Emergency Med Ctr, Dept Gastroenterol & Hepatol, Chongqing, Peoples R China; [Yu, Ting] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing, Peoples R China; [Lai, Xiangyu; He, Yongpeng] Chongqing Univ, Chongqing Canc Hosp, Chongqing Canc Inst, Canc Hosp,Chongqing Key Lab Translat Res Canc Meta, Chongqing, Peoples R China"

通信作者："Lai, XY; He, YP (通讯作者)，Chongqing Univ, Chongqing Canc Hosp, Chongqing Canc Inst, Canc Hosp,Chongqing Key Lab Translat Res Canc Meta, Chongqing, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001195895900001

JCR分区：Q2

影响因子：4

年份：2024

卷号：15

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Lacticaseibacillus rhamnosus; colon cancer; azoxymethane; mRNA; mice

摘要："Introduction: Lacticaseibacillus rhamnosus AFY06 (LR-AFY06) is a microorganism isolated from naturally fermented yogurt in Xinjiang, China. Methods: In this study, we investigated the effects and mechanisms of LR-AFY06 in a mouse model of inflammation-associated colon cancer. The mouse model was established by azoxymethane/dextran sulfate sodium (AOM/DSS) induction. The tumor number in intestinal tissues was counted, and the histopathological analysis was performed on colon tissues. Enzyme-linked immunosorbent assay and real-time quantitative polymerase chain reaction were performed to measure relevant protein levels in colon tissues. Results: LR-AFY06 treatment alleviated weight loss, increased organ index, reduced intestinal tumor incidence, improved histopathological damage, decreased the levels of inflammatory cytokines such as interleukin-6 (IL-6), interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF-alpha), nuclear factor kappa B (NF-kappa B), and inducible nitric oxide synthase (iNOS) in the serum and colon tissue, downregulated the mRNA expression of inhibitor of NF-kappa B beta (I kappa B beta), p65, p50, p52, B-cell lymphoma-2 (Bcl-2), and B-cell lymphoma-extra large (Bcl-xL) in colon tissues, and increased the mRNA expression of Bid and caspase-8. The high concentration of LR-AFY06 exerted a better effect than the low concentration; however, the effect was slightly inferior to that of aspirin. Moreover, LR-AFY06 mitigated the intestinal inflammatory process and inhibited intestinal tumor development by regulating the NF-kappa B and apoptosis pathways. Discussion: The present study indicates the regulatory potential of LR-AFY06 in inflammation-associated colorectal cancer in mice, providing a valuable basis for further research."

基金机构：Scientific Research Project of Chongqing Medical Biotechnology Association [cmba2022kyym-zkxmQ0012]; Natural Science Foundation of Chongqing [CSTB2023NSCQ-BHX0158]

基金资助正文："The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the Scientific Research Project of Chongqing Medical Biotechnology Association (cmba2022kyym-zkxmQ0012) and the Natural Science Foundation of Chongqing (CSTB2023NSCQ-BHX0158)."