Importance of DNA nanotechnology for DNA methyltransferases in biosensing assays

作者全名："Huang, Yuqi; Zhao, Zixin; Yi, Gang; Zhang, Mingjun"

作者地址："[Huang, Yuqi; Zhang, Mingjun] Chongqing Jiulongpo Dist Peoples Hosp, Clin Lab, Chongqing 400050, Peoples R China; [Zhao, Zixin; Yi, Gang] Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Zhang, MJ (通讯作者)，Chongqing Jiulongpo Dist Peoples Hosp, Clin Lab, Chongqing 400050, Peoples R China.; Yi, G (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost Minist Educ, Chongqing 400016, Peoples R China."

来源：JOURNAL OF MATERIALS CHEMISTRY B

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001196404300001

JCR分区：Q1

影响因子：6.1

年份：2024

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Review; Early Access

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摘要："DNA methylation is the process by which specific bases on a DNA sequence acquire methyl groups under the catalytic action of DNA methyltransferases (DNMT). Abnormal changes in the function of DNMT are important markers for cancers and other diseases; therefore, the detection of DNMT and the selection of its inhibitors are critical to biomedical research and clinical practice. DNA molecules can undergo intermolecular assembly to produce functional aggregates because of their inherently stable physical and chemical properties and unique structures. Conventional DNMT detection methods are cumbersome and complicated processes; therefore, it is necessary to develop biosensing technology based on the assembly of DNA nanostructures to achieve rapid analysis, simple operation, and high sensitivity. The design of the relevant program has been employed in life science, anticancer drug screening, and clinical diagnostics. In this review, we explore how DNA assembly, including 2D techniques like hybridization chain reaction (HCR), rolling circle amplification (RCA), catalytic hairpin assembly (CHA), and exponential isothermal amplified strand displacement reaction (EXPAR), as well as 3D structures such as DNA tetrahedra, G-quadruplexes, DNA hydrogels, and DNA origami, enhances DNMT detection. We highlight the benefits of these DNA nanostructure-based biosensing technologies for clinical use and critically examine the challenges of standardizing these methods. We aim to provide reference values for the application of these techniques in DNMT analysis and early cancer diagnosis and treatment, and to alert researchers to challenges in clinical application. We explore how DNA assembly, including 2D techniques, as well as 3D structures, enhances DNA methyltransferases detection. We highlight the benefits of these DNA nanostructure-based biosensing technologies for clinical use and examine the challenges of standardizing these methods."

基金机构：Natural Science Foundation of Chongqing Municipality [CSTB2022NSCQ-MSX0892]; Chongqing Natural Science Foundation [2023-03-006-Z]; Jiulongpo district-level technology foresight and system innovation project

基金资助正文：This work was supported by Chongqing Natural Science Foundation (grant no. CSTB2022NSCQ-MSX0892) and Jiulongpo district-level technology foresight and system innovation project (grant no. 2023-03-006-Z).