Nr5a2 ensures inner cell mass formation in mouse blastocyst

作者全名："Zhao, Yanhua; Zhang, Meiting; Liu, Jiqiang; Hu, Xinglin; Sun, Yuchen; Huang, Xingwei; Li, Jingyu; Lei, Lei"

作者地址："[Zhao, Yanhua; Zhang, Meiting; Liu, Jiqiang; Hu, Xinglin; Sun, Yuchen; Huang, Xingwei; Lei, Lei] Harbin Med Univ, Dept Histol & Embryol, Harbin 150081, Peoples R China; [Li, Jingyu] Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Women & Childrens Hosp, Chongqing 400010, Peoples R China"

通信作者："Lei, L (通讯作者)，Harbin Med Univ, Dept Histol & Embryol, Harbin 150081, Peoples R China.; Li, JY (通讯作者)，Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Women & Childrens Hosp, Chongqing 400010, Peoples R China."

来源：CELL REPORTS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001197676300001

JCR分区：Q1

影响因子：7.5

年份：2024

卷号：43

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Recent studies have elucidated Nr5a2's role in activating zygotic genes during early mouse embryonic development. Subsequent research, however, reveals that Nr5a2 is not critical for zygotic genome activation but is vital for the gene program between the 4- and 8 -cell stages. A significant gap exists in experimental evidence regarding its function during the first lineage differentiation's pivotal period. In this study, we observed that approximately 20% of embryos developed to the blastocyst stage following Nr5a2 ablation. However, these blastocysts lacked inner cell mass (ICM), highlighting Nr5a2's importance in first lineage differentiation. Mechanistically, using RNA sequencing and CUT&Tag, we found that Nr5a2 transcriptionally regulates ICM-specific genes, such as Oct4, to establish the pluripotent network. Interference with or overexpression of Nr5a2 in single blastomeres of 2 -cell embryos can alter the fate of daughter cells. Our results indicate that Nr5a2 works as a doorkeeper to ensure ICM formation in mouse blastocyst."

基金机构：Key Projects of Heilongjiang Natural Science Foundation [MSX0875]; [ZD2021C005]

基金资助正文："We thank Wang Jiaqiang (Northeast Agricultural University) for helpful discus-sions and linguistic assistance. We would like to express our gratitude to BioRender for providing a user-friendly platform that facilitated the creation of the graphical abstract used in this study, enhancing the visual representation of our research findings. We would like to express our sincere gratitude to the National Natural Science Foundation of China (grant no. 82301880), the Nature Science Foundation of Chongqing (grant no. CSTB2022NSCQ-MSX0875) , and the Key Projects of Heilongjiang Natural Science Foundation (grant no. ZD2021C005) for their generous financial support.r MSX0875) , and the Key Projects of Heilongjiang Natural Science Foundation (grant no. ZD2021C005) for their generous financial support."