A study on expression of GRP78 and CHOP in neutrophil endoplasmic reticulum and their relationship with neutrophil apoptosis in the development of sepsis

作者全名："Zhao, Jinchuan; Yang, Ping; Lu, Li; Yi, Tao; Li, Yunfeng; Mao, Wei; Zhou, Qin; Lin, Ke"

作者地址："[Zhao, Jinchuan] Chongqing Emergency Med Ctr, Emergency Dept, Chongqing, Peoples R China; [Yang, Ping] Chongqing Med Univ, Intens Care Unit, Affiliated Hosp 2, Chongqing, Peoples R China; [Lu, Li; Yi, Tao; Li, Yunfeng; Mao, Wei; Zhou, Qin; Lin, Ke] Chongqing Med Univ, Univ Town Hosp, Emergency Dept, Chongqing, Peoples R China"

通信作者："Lin, K (通讯作者)，Chongqing Med Univ, Univ Town Hosp, Emergency Dept, Chongqing, Peoples R China."

来源：JOURNAL OF BIOSCIENCES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001198521700001

JCR分区：Q2

影响因子：2.1

年份：2024

卷号：49

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：Cell apoptosis; endoplasmic reticulum stress; neutrophils; pathogenesis; sepsis

摘要："We investigated the relationship between neutrophil apoptosis and endoplasmic reticulum stress (ERS) in sepsis and its mechanism. A prospective cohort study was conducted by recruiting a total of 58 patients with sepsis. Peripheral blood samples were collected on 1, 3, 5 and 7 days after admission to the ICU. The expressions of endoplasmic reticulum specific glucose regulatory protein 78 (GRP78), C/EBP homologous protein (CHOP), apoptosis signal-regulating kinase 1 (ASK1), Bcl-2-like 11 (BIM), death receptor 5 (DR5), c-Jun N-terminal kinases (JNK) and p38 were detected by Western blot and PCR. The subcellular location of CHOP and GRP78 was observed by immunofluorescence analysis. Spearman correlation was used to analyze the correlation between the expression of chop protein and the apoptosis rate of peripheral blood neutrophils. Healthy volunteers in the same period were selected as the healthy control group. The expression of GRP78 protein was significantly elevated on the first day of ICU admission and showed a decreasing trend on the third, fifth and seventh day, but was significantly higher than the corresponding healthy control group. The expression of CHOP protein reached the highest level on the third day. The expression of chop protein in each group was significantly higher than that in the corresponding healthy control group. Immunofluorescence staining clearly showed that the CHOP protein accumulated in the nucleus, with an elevation in the intensity of GRP78. The neutrophil apoptosis rate of sepsis patients on the 1st, 3rd, 5th and 7th day of ICU stay was significantly higher than that of the healthy control group, with the highest apoptosis rate on the 3rd day, and then decreased gradually. CHOP protein expression level was significantly positively correlated with neutrophil apoptosis rate in sepsis patients. Endoplasmic reticulum stress occurs in neutrophils during the development of sepsis. GRP78 protein and CHOP protein may be involved in the pathological process of neutrophil apoptosis in sepsis."

基金机构：Medical research project of Chongqing Health and Family Planning Commission; Chongqing Shapingba District Decision Making Consultation and Management Project [Jcd202137]; [2016MSXM079]

基金资助正文：This work was supported by a medical research project of Chongqing Health and Family Planning Commission (2016MSXM079) and Chongqing Shapingba District Decision Making Consultation and Management Project (Jcd202137).