Determination of coumarin-3-carboxylic acid in the liver and heart tissue of Sprague-Dawley rats after intragastric administration of extractive of leaves of F. virens var. sublanceolata by HPLC

作者全名：Xiang, Yujie; Chuahe, Bahetibieke; Zhang, Jing; Li, Jiansha; Jiang, Xinhui

作者地址：[Xiang, Yujie; Zhang, Jing; Jiang, Xinhui] Chongqing Med Univ, Chongqing Res Ctr Pharmaceut Engn, Sch Pharm, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Chuahe, Bahetibieke] Agr & Rural Bur Habahe Cty, Xinjiang 836700, Peoples R China; [Li, Jiansha] Jiangsu Hengrui Pharmaceut Co Ltd, Chongqing 400042, Peoples R China

通信作者：Jiang, XH (通讯作者)，Chongqing Med Univ, Chongqing Res Ctr Pharmaceut Engn, Sch Pharm, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China.

来源：ACTA CHROMATOGRAPHICA

ESI学科分类：CHEMISTRY

WOS号：WOS:001198885400007

JCR分区：Q3

影响因子：1.7

年份：2024

卷号：36

期号：1

开始页：1

结束页：6

文献类型：Article

关键词：leaves of F. virens var. sublanceolata; total flavonoids extraction; coumarin-3-carboxylic acid; HPLC; liver tissue; heart tissue

摘要：The study was aimed to validate and optimize high performance liquid chromatographic (HPLC) method for the determination of coumarin-3-carboxylic acid (C3A) in the heart and liver issue of Sprague-Dawley (SD) rats after intragastric administration of extractive of leaves of Ficus virens var sublanceolata. And simple ADME and target prediction analyses were performed for C3A. Ethyl acetate was employed to precipitate protein with appropriate sensitivity and acceptable matrix effects. The satisfactory separation was developed on an ODS2 column (4.6 mm 3 250 mm, 5 mu m) by gradient elution with a methanol-acetic acid solution (pH 5 3.0) as the mobile phase. The flow rate was 1.0 mL min-1, the column temperature was maintained at 30 +/- 2 8C, the injection volume was 20 mu L, and the detection wavelength was set as 309 nm. The method was fully validated in terms of selectivity, linearity, accuracy, precision, extraction recovery and stability. The results of the ADME analysis found that C3A has excellent characteristics of drug-likeness, consistent with good bio-absorption. And the predicted 12 target protein belongs to the amine oxidoreductase and carbonic anhydrase target class. This method is simple, rapid, sensitive, and accurate for the determination of coumarin-3-carboxylic acid in the heart and liver tissue of SD rats.

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