Dysbiosis of gut microbiota and metabolites is associated with radiation-induced colorectal fibrosis and is restored by adipose-derived mesenchymal stem cell therapy
作者全名："Thandar, Mya; Yang, Xiaojie; Zhu, Yuanchang; Zhang, Xueying; Chen, Zhifen; Huang, Shenghui; Chi, Pan"
作者地址："[Thandar, Mya; Zhu, Yuanchang; Zhang, Xueying; Chen, Zhifen; Huang, Shenghui; Chi, Pan] Fujian Med Univ, Union Hosp, Dept Colorectal Surg, Fuzhou 350001, Fujian Province, Peoples R China; [Thandar, Mya; Yang, Xiaojie; Zhu, Yuanchang; Zhang, Xueying; Chi, Pan] Fujian Med Univ, Dept Colorectal Surg, Key Lab, Minist Educ Gastrointestinal Canc, Fuzhou 350001, Fujian Province, Peoples R China; [Yang, Xiaojie] Chongqing Med Univ, Dept Thorac Surg, Affiliated Hosp 3, Chongqing 401100, Peoples R China; [Chen, Zhifen; Huang, Shenghui; Chi, Pan] Fujian Med Univ, Training Ctr Minimally Invas Surg, Union Hosp, Fuzhou 350001, Fujian Province, Peoples R China; [Chi, Pan] Fujian Med Univ, Dept Otolaryngol, Union Hosp, 29 Xinquan Rd, Fuzhou 350001, Fujian Province, Peoples R China"
通信作者："Chen, ZF; Huang, SH; Chi, P (通讯作者)，Fujian Med Univ, Union Hosp, Dept Colorectal Surg, Fuzhou 350001, Fujian Province, Peoples R China.; Chi, P (通讯作者)，Fujian Med Univ, Dept Otolaryngol, Union Hosp, 29 Xinquan Rd, Fuzhou 350001, Fujian Province, Peoples R China."
来源：LIFE SCIENCES
ESI学科分类：BIOLOGY & BIOCHEMISTRY
WOS号：WOS:001199292900001
JCR分区：Q1
影响因子：5.2
年份：2024
卷号：341
期号：
开始页：
结束页：
文献类型：Article
关键词：Radiation -induced colorectal fibrosis; Adipose -derived mesenchymal stem cells; Gut microbiota; Metabolites; Bacteroides; Camelledionol
摘要："Aims: This study aimed to investigate the effects of adipose-derived mesenchymal stem cells (ADSCs) on radiation-induced colorectal fibrosis (RICF) along with the associated dysbiosis of gut microbiota and metabolites. Main methods: Fecal microbiota were assessed through 16S rRNA gene sequencing, and the fecal metabolome was characterized using liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. The correlation between microbiota and metabolome data was explored. Key findings: ADSC injection demonstrated a significant restoration of radiation-induced intestinal damage in vivo. At the phylum level, irradiated rats exhibited an increase in Bacteroidota and Campilobacterota, and a decrease in Firmicutes and Desulfobacterota, contrasting with the ADSC treatment group. Metabolomic analysis revealed 72 differently expressed metabolites (DEMs) from gas chromatography-mass spectrometry and 284 DEMs from liquid chromatography-mass spectrometry in the radiation group compared to the blank group. In the ADSC treatment group versus the radiation group, 36 DEMs from gas chromatography-mass spectrometry and 341 DEMs from liquid chromatography-mass spectrometry were identified. KEGG enrichment analysis implicated pathways such as steroid hormone biosynthesis, gap junction, primary bile acid biosynthesis, citrate cycle, cAMP signaling pathway, and alanine, aspartate, and glutamate metabolism during RICF progression and after treated with ADSCs. Correlation analysis highlighted the role of ADSCs in modulating the metabolic process of Camelledionol in fecal Bacteroides. Significance: These findings underscore the potential of ADSCs in reversing dysbiosis and restoring normal colonic flora in the context of RICF, offering valuable insights for therapeutic interventions targeting radiation-induced complications."
基金机构：Fujian Provincial Science and Technology Innovation Joint Fund Project [2022J01753]; Fujian Provincial Health Tech-nology Project [2019Y92050173]; [2020GGB022]
基金资助正文：This work was supported by Natural Science Foundation of Fujian Province (2022J01726) ; Natural Science Foundation of Fujian Provincer (2022J01753) ; Fujian Provincial Science and Technology Innovation Joint Fund Project (2019Y92050173) ; Fujian Provincial Health Tech-nology Project (2020GGB022) .
