Silk Fibroin Films with Microscopic Surface Pattern as Promising Cartilage Tissue Engineering Scaffold in Supporting MSCs Amplification and Chondrogenic Differentiation

作者全名："Wang, Xin; Yang, Junjun; Wang, Rong; Huang, Yumengfei; Wan, Zu; Wang, Zhi; Fu, Zhenlan; Chen, Cheng; Chen, Guangxing; Gong, Xiaoyuan"

作者地址："[Wang, Xin; Wang, Rong; Wan, Zu; Wang, Zhi; Fu, Zhenlan; Chen, Guangxing; Gong, Xiaoyuan] Army Med Univ, Mil Med Univ 3, Southwest Hosp, Ctr Joint Surg, 30 Gaotanyan Main St, Chongqing 400038, Peoples R China; [Yang, Junjun; Huang, Yumengfei] Chongqing Univ, Minist Educ, Key Lab Biorheol Sci & Technol, Coll Bioengn, Chongqing, Peoples R China; [Chen, Cheng] Chongqing Med Univ, Coll Med Informat, 1 Yixueyuan St, Chongqing 400016, Peoples R China"

通信作者："Chen, GX; Gong, XY (通讯作者)，Army Med Univ, Mil Med Univ 3, Southwest Hosp, Ctr Joint Surg, 30 Gaotanyan Main St, Chongqing 400038, Peoples R China.; Chen, C (通讯作者)，Chongqing Med Univ, Coll Med Informat, 1 Yixueyuan St, Chongqing 400016, Peoples R China."

来源：JOURNAL OF NATURAL FIBERS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001199398000001

JCR分区：Q1

影响因子：2.8

年份：2024

卷号：21

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Cartilage repair; stem cell; chondrogenic differentiation; silk fibroin; microscopic surface; film

摘要："Mesenchymal stem cells (MSCs) with multipotential differentiation ability are main cell source for cartilage tissue engineering, but its application is restricted by the difficulty in regulating committed chondrogenic differentiation. Study revealed that certain substrate topographic structure can enhance the chondrogenic differentiation of MSCs. This study prepared silk fibroin (SF) films with gradient microscopic surface patterns based on P280-P7000 type specification sandpapers, and investigated the adhesion, proliferation and chondrogenic differentiation of human adipose-derived stem cells (hADSCs) on the SF films. The results showed that the SF films surface had convex dots with diameter of 3-50 mu m, and hADSCs could adhere and proliferate well on the film. Transcriptomics analysis indicated that hADSCs tend to differentiate differently on the SF films, among which hADSCs on P1000 SF films had higher expression of chondrogenic markers Sox-9 and Col-II, with activated yes associated protein (YAP) and restricted F-actin. In vitro co-culture of human OA knee cartilage with hADSCs seeded SF films found satisfactory cartilage protection effect in P1000 group. Overall, this study preliminarily revealed the microscopic surface pattern of SF films may induce chondrogenic differentiation of hADSCs through extracellular matrix mediated Hippo/Yap pathway, and provided a new strategy for cartilage repair."

基金机构："Chongqing Natural Science Foundation General Project [cstc2021jcyj-msxmX0135, CSTB2022NSCQ-MSX0139]; Chongqing Natural Science Foundation Innovation Development Joint Fund [Municipal Education Commission] Project [2022NSCQ-LZX0066]; Chongqing Postdoctoral Research Project Special Fund [2021XM3033]; Postdoctoral Science Fund Project of the Chongqing Natural Science Foundation [CSTB2023NSCQ-BHX0156]"

基金资助正文："This work was supported by Chongqing Natural Science Foundation General Project [cstc2021jcyj-msxmX0135 and CSTB2022NSCQ-MSX0139], Chongqing Natural Science Foundation Innovation Development Joint Fund [Municipal Education Commission] Project [2022NSCQ-LZX0066], Chongqing Postdoctoral Research Project Special Fund [2021XM3033], and Postdoctoral Science Fund Project of the Chongqing Natural Science Foundation [CSTB2023NSCQ-BHX0156]."