The Expression of MAFB Gene in Circulating Monocytes Is Related to Chronic Inflammatory Status in T2DM Patients

作者全名："Zhang, Wanliang; Chen, Wenyun; Lei, Jingwei; Li, Jie; Yang, Mengliu; Li, Ling"

作者地址："[Zhang, Wanliang; Chen, Wenyun; Lei, Jingwei; Li, Jie; Li, Ling] Chongqing Med Univ, Coll Lab Med, Dept Clin Biochem, Key Lab Lab Med Diagnost,Minist Educ, Chongqing, Peoples R China; [Zhang, Wanliang; Chen, Wenyun; Lei, Jingwei; Li, Jie; Li, Ling] Chongqing Med Univ, Coll Lab Med, Dept Clin Biochem, Chongqing, Peoples R China; [Yang, Mengliu] Chongqing Med Univ, Affiliated Hosp 2, Dept Endocrinol, Chongqing, Peoples R China"

通信作者："Li, L (通讯作者)，Chongqing Med Univ, Coll Lab Med, Dept Clin Biochem, Key Lab Lab Med Diagnost,Minist Educ, Chongqing, Peoples R China.; Li, L (通讯作者)，Chongqing Med Univ, Coll Lab Med, Dept Clin Biochem, Chongqing, Peoples R China."

来源：INFLAMMATION

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001200345400001

JCR分区：Q2

影响因子：4.5

年份：2024

卷号：　

期号：　

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结束页：　

文献类型：Article; Early Access

关键词：MAFB; T2DM; chronic inflammation; PBMCs.

摘要："Immune cell-mediated chronic inflammation is one of the causes of type 2 diabetes mellitus (T2DM). Therefore, identifying inflammatory markers in circulating immune cells is highly important for predicting insulin resistance (IR) and the occurrence of T2DM. In this study, we discovered that differentially expressed genes (DEGs) in peripheral blood mononuclear cells (PBMCs) from T2DM patients were associated with innate immunity and chronic inflammatory responses through bulk transcriptome sequencing (bulk RNA-seq). Gene integration analysis revealed that nine DEGs were upregulated, and receiver operating characteristic (ROC) curve analysis revealed that V-maf musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB), a candidate biomarker, has a certain predictive value for T2DM. In population-based cohort studies, we found that MAFB expression was significantly upregulated in the PBMCs of T2DM patients and was significantly correlated with homeostasis model assessment of IR (HOMA-IR), tumor necrosis factor-alpha (TNF-alpha), adiponectin (Adipoq), etc. We further evaluated the sensitivity and specificity of MAFB and other clinical parameters for predicting and diagnosing T2DM and found that MAFB expression in PBMCs had a positive effect on the prediction and diagnosis of T2DM. Finally, single-cell RNA sequencing (scRNA-seq) analysis revealed that the increase in MAFB expression was mainly in nonclassical monocytes. Our results suggest that increased MAFB expression in circulating monocytes may mediate chronic inflammatory status in patients with T2DM. Therefore, MAFB gene expression in circulating monocytes has certain clinical significance for predicting and assisting in the diagnosis of T2DM."

基金机构："China Postdoctoral Science Foundation; Department of Endocrinology of the Second Affiliated Hospital of Chongqing Medical University; Key Laboratory of Clinical Laboratory Diagnostics of Ministry of Education, College of Laboratory Medicine, Chongqing Medical University"

基金资助正文："The authors would like to thank all the participants for their participation in this study. We thank the Department of Endocrinology of the Second Affiliated Hospital of Chongqing Medical University for providing professional guidance. The authors thank the professional platform provided by the Key Laboratory of Clinical Laboratory Diagnostics of Ministry of Education, College of Laboratory Medicine, Chongqing Medical University."