Scaling up of a Self-Confined Catalytic Hybridization Circuit for Robust microRNA Imaging

作者全名：Gong, Xue; Li, Ruomeng; Zhang, Jiajia; Zhang, Pu; Jiang, Zhongwei; Hu, Lianzhe; Liu, Xiaoqing; Wang, Yi; Wang, Fuan

作者地址：[Gong, Xue; Li, Ruomeng; Liu, Xiaoqing; Wang, Fuan] Wuhan Univ, Zhongnan Hosp, Coll Chem & Mol Sci, Dept Gastroenterol, Wuhan 430072, Peoples R China; [Gong, Xue; Zhang, Jiajia; Jiang, Zhongwei; Hu, Lianzhe; Wang, Yi] Chongqing Normal Univ, Coll Chem, Chongqing Key Lab Green Catalysis Mat & Technol, Engn Res Ctr Biotechnol Act Subst,Minist Educ, Chongqing 401331, Peoples R China; [Zhang, Pu] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China

通信作者：Wang, F (通讯作者)，Wuhan Univ, Zhongnan Hosp, Coll Chem & Mol Sci, Dept Gastroenterol, Wuhan 430072, Peoples R China.; Wang, Y (通讯作者)，Chongqing Normal Univ, Coll Chem, Chongqing Key Lab Green Catalysis Mat & Technol, Engn Res Ctr Biotechnol Act Subst,Minist Educ, Chongqing 401331, Peoples R China.

来源：ADVANCED SCIENCE

ESI学科分类：PHYSICS

WOS号：WOS:001201441500001

JCR分区：Q1

影响因子：14.3

年份：2024

卷号：11

期号：22

开始页：　

结束页：　

文献类型：Article

关键词：catalytic DNA circuit; diagnosis; DNA nanotechnology; imaging; self-assembly

摘要：The precise regulation of cellular behaviors within a confined, crowded intracellular environment is highly amenable in diagnostics and therapeutics. While synthetic circuitry system through a concatenated chemical reaction network has rarely been reported to mimic dynamic self-assembly system. Herein, a catalytic self-defined circuit (CSC) for the hierarchically concatenated assembly of DNA domino nanostructures is engineered. By incorporating pre-sealed symmetrical fragments into the preying hairpin reactants, the CSC system allows the hierarchical DNA self-assembly via a microRNA (miRNA)-powered self-sorting catalytic hybridization reaction. With minimal strand complexity, this self-sustainable CSC system streamlined the circuit component and achieved localization-intensified cascaded signal amplification. Profiting from the self-adaptively concatenated hybridization reaction, a reliable and robust method has been achieved for discriminating carcinoma tissues from the corresponding para-carcinoma tissues. The CSC-sustained self-assembly strategy provides a comprehensive and smart toolbox for organizing various hierarchical DNA nanostructures, which may facilitate more insights for clinical diagnosis and therapeutic assessment. A catalytic self-defined circuit (CSC), facilitating hierarchical-dependent self-assembly of DNA nanostructure, is developed for robust and reliable discriminating carcinoma tissues and the corresponding para-carcinoma tissues in clinic samples. image

基金机构：National Natural Science Foundation of China; Natural Science Foundation of Chongqing [CSTB2022NSCQ-MSX0466, cstc2021ycjh-bgzxm0113]; Natural Science Foundation Joint Fund for Innovation and Development of Chongqing Municipal Education Commission [CSTB2022NSCQ-LZX0077]; Science and Technology Research Project of Chongqing Municipal Education Commission [KJQN202200559]; Chongqing Innovation Research Group Project [CXQT21015]; Startup Fund of Chongqing Normal University [22XLB010]; Chongqing Youth Expert Studio; [22204012]; [81972020]; [22074112]

基金资助正文：X.G. and R.L. contributed equally to this work. This work was supported by the National Natural Science Foundation of China (22204012, 81972020, and 22074112), the Natural Science Foundation of Chongqing (CSTB2022NSCQ-MSX0466 and cstc2021ycjh-bgzxm0113), the Natural Science Foundation Joint Fund for Innovation and Development of Chongqing Municipal Education Commission (CSTB2022NSCQ-LZX0077), the Science and Technology Research Project of Chongqing Municipal Education Commission (KJQN202200559), the Chongqing Innovation Research Group Project (CXQT21015), and the Startup Fund of Chongqing Normal University (22XLB010). Y.W. was also sponsored by the Chongqing Youth Expert Studio.