Combination of Fushengong decoction with Western medicine on patients with chronic renal failure: An observational study
作者全名：Zou, Bo; Yuan, Qiaoqiao; Luo, Hongyu; Wang, Munan; Chen, Xin; Gao, Zuling; Wang, Jianwei; Peng, Yongbo; Yang, Haijun; Dai, Feng; Huang, Xuekuan
作者地址：[Zou, Bo; Yuan, Qiaoqiao; Gao, Zuling; Yang, Haijun; Dai, Feng] Chongqing Med Univ, Yongchuan Tradit Chinese Med, Dept Nephrol, Chongqing, Peoples R China; [Zou, Bo; Luo, Hongyu; Wang, Munan; Chen, Xin; Wang, Jianwei; Huang, Xuekuan] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing Key Lab Tradit Chinese Med Prevent & Cur, Chongqing, Peoples R China; [Peng, Yongbo] Chongqing Med Univ, Coll Pharm, Chongqing Key Lab Pharmaceut Metab Res, Key Lab Biochem & Mol Pharmacol, Chongqing, Peoples R China; [Huang, Xuekuan] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing Key Lab Tradit Chinese Med Prevent & Cur, Chongqing 400016, Peoples R China
通信作者：Huang, XK (通讯作者)，Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing Key Lab Tradit Chinese Med Prevent & Cur, Chongqing 400016, Peoples R China.
来源：MEDICINE
ESI学科分类：CLINICAL MEDICINE
WOS号：WOS:001201698900026
JCR分区：Q2
影响因子：1.3
年份：2024
卷号：103
期号：15
开始页：
结束页：
文献类型：Article
关键词：bioinformatics analysis; chronic renal failure; Fushengong decoction; iTRAQ; LC-MS/MS
摘要：Chronic renal failure (CRF) causes a reduction in glomerular filtration rate and damage to renal parenchyma. Fushengong decoction (FSGD) showed improvement in renal function in CRF rats. This study aims to analyze the differentially expressed proteins in CRF patients treated with Western medicine alone or in combination with FSGD. Sixty patients with CRF recruited from Yongchuan Traditional Chinese Medicine Hospital affiliated to Chongqing Medical University were randomly assigned into control (treated with Western medicine alone) and observation groups (received additional FSGD treatment thrice daily for 8 weeks). The clinical efficacy and changes in serum Bun, serum creatinine, Cystatin C, and transforming growth factor beta 1 (TGF-beta 1) before and after treatment were observed. We employed isotope relative labeling absolute quantification labeling and liquid chromatography-mass spectrometry to identify differentially expressed proteins and carried out bioinformatics Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. Patients in the observation group showed greater clinical improvement and lower levels of serum Bun, serum creatinine, Cyc-c, and TGF-beta 1 than the control group. We identified 32 differentially up-regulated and 52 down-regulated proteins in the observation group. These proteins are involved in the blood coagulation system, protein serine/threonine kinase activity, and TGF-beta, which are closely related to the pathogenesis of CRF. Protein-protein-interaction network analysis indicated that candidate proteins fibronectin 1, fibrinogen alpha chain, vitronectin, and Serpin Family C Member 1 were in the key nodes. This study provided an experimental basis suggesting that FSGD combined with Western medicine could significantly improve renal function and renal fibrosis of CRF patients, which may be through the regulation of fibronectin 1, fibrinogen alpha chain, vitronectin, Serpin Family C Member 1, TGF-beta, and the complement coagulation pathway (see Graphical abstract S1, Supplemental Digital Content, http://links.lww.com/MD/L947).
基金机构：Chongqing Health and Family Planning Commission, Chongqing Science and Technology Commission [ZY201802149]; Chongqing Technical Innovation and Application Development Special Project [CSTC2019JSCX-MSXMX0119]; Discipline Talents Program of School of Pharmacy and Innovate Start-up Project in Chongqing Medicine University [YXY2021XSZ04]; National Natural Science Foundation of China [81202896]; Natural Science Foundation Project of CQ CSTC [cstc2018jcyjAX0460]
基金资助正文：This work was supported by the Chongqing Health and Family Planning Commission, Chongqing Science and Technology Commission [ZY201802149], Chongqing Technical Innovation and Application Development Special Project [CSTC2019JSCX-MSXMX0119], Discipline Talents Program of School of Pharmacy and Innovate Start-up Project in Chongqing Medicine University [YXY2021XSZ04], the National Natural Science Foundation of China [81202896], and the Natural Science Foundation Project of CQ CSTC [cstc2018jcyjAX0460].
