Macrophage membrane-camouflaged pH-sensitive nanoparticles for targeted therapy of oral squamous cell carcinoma

作者全名：Yang, Lin; Li, Hongjiao; Luo, Aihua; Zhang, Yao; Chen, Hong; Zhu, Li; Yang, Deqin

作者地址：[Yang, Lin; Li, Hongjiao; Luo, Aihua; Zhang, Yao; Chen, Hong; Yang, Deqin] Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing 404100, Peoples R China; [Yang, Lin; Li, Hongjiao; Luo, Aihua; Zhang, Yao; Chen, Hong; Yang, Deqin] Chongqing Med Univ, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 404100, Peoples R China; [Yang, Lin; Li, Hongjiao; Luo, Aihua; Zhang, Yao; Chen, Hong; Yang, Deqin] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 404100, Peoples R China; [Yang, Lin; Li, Hongjiao; Luo, Aihua; Chen, Hong; Yang, Deqin] Chongqing Key Lab Oral Dis & Biomed Sci, 426 Songshi North Rd, Chongqing 401147, Peoples R China; [Zhu, Li] Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400044, Peoples R China

通信作者：Yang, DQ (通讯作者)，Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing 404100, Peoples R China.; Yang, DQ (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 404100, Peoples R China.; Yang, DQ (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 404100, Peoples R China.; Yang, DQ (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, 426 Songshi North Rd, Chongqing 401147, Peoples R China.; Zhu, L (通讯作者)，Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400044, Peoples R China.

来源：JOURNAL OF NANOBIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001201852900003

JCR分区：Q1

影响因子：10.6

年份：2024

卷号：22

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Oral squamous cell carcinoma; pH-sensitive; Macrophage membrane; Target delivery

摘要：Background Oral cancer is the most common malignant tumor of the head and neck, and 90% of cases are oral squamous cell carcinoma (OSCC). Chemotherapy is an important component of comprehensive treatment for OSCC. However, the clinical treatment effect of chemotherapy drugs, such as doxorubicin (DOX), is limited due to the lack of tumor targeting and rapid clearance by the immune system. Thus, based on the tumor-targeting and immune evasion abilities of macrophages, macrophage membrane-encapsulated poly(methyl vinyl ether alt maleic anhydride)-phenylboronic acid-doxorubicin nanoparticles (MM@PMVEMA-PBA-DOX NPs), briefly as MM@DOX NPs, were designed to target OSCC. The boronate ester bonds between PBA and DOX responded to the low pH value in the tumor microenvironment, selectively releasing the loaded DOX.Results The results showed that MM@DOX NPs exhibited uniform particle size and typical core-shell structure. As the pH decreased from 7.4 to 5.5, drug release increased from 14 to 21%. The in vitro targeting ability, immune evasion ability, and cytotoxicity of MM@DOX NPs were verified in HN6 and SCC15 cell lines. Compared to free DOX, flow cytometry and fluorescence images demonstrated higher uptake of MM@DOX NPs by tumor cells and lower uptake by macrophages. Cell toxicity and live/dead staining experiments showed that MM@DOX NPs exhibited stronger in vitro antitumor effects than free DOX. The targeting and therapeutic effects were further confirmed in vivo. Based on in vivo biodistribution of the nanoparticles, the accumulation of MM@DOX NPs at the tumor site was increased. The pharmacokinetic results demonstrated a longer half-life of 9.26 h for MM@DOX NPs compared to 1.94 h for free DOX. Moreover, MM@DOX NPs exhibited stronger tumor suppression effects in HN6 tumor-bearing mice and good biocompatibility.Conclusions Therefore, MM@DOX NPs is a safe and efficient therapeutic platform for OSCC.

基金机构：Natural Science Foundation Project of Chongqing Science and Technology Commission

基金资助正文：Not applicable.