Preparation of etoposide liposomes for enhancing antitumor efficacy on small cell lung cancer and reducing hematotoxicity of drugs

作者全名：Huang, Ruixue; Chen, Huali; Pi, Damao; He, Xuemei; Yu, Chao; Yu, Chaoqun

作者地址：[Huang, Ruixue; Chen, Huali; Yu, Chao; Yu, Chaoqun] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmaceut Preparat & Nanomed, Chongqing 400016, Peoples R China; [Pi, Damao] Chongqing Med Univ, Affiliated Hosp 1, Dept Pharm, Chongqing 400016, Peoples R China; [He, Xuemei] Chongqing Med Univ, Affiliated Hosp 1, Dept Ultrasound, Chongqing 400016, Peoples R China; [Yu, Chao] Chongqing Med Univ, Coll Pharm 10, Chongqing Key Lab Pharmaceut Metab Res, Chongqing 400016, Peoples R China; [Yu, Chao] Chongqing Med Univ, Coll Pharm, Pharmaceut Engn Res Ctr, Chongqing 400016, Peoples R China

通信作者：Yu, CQ (通讯作者)，Chongqing Med Univ, Coll Pharm, Res Ctr Pharmaceut Preparat & Nanomed, Chongqing 400016, Peoples R China.

来源：EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001202538200001

JCR分区：Q1

影响因子：4.4

年份：2024

卷号：198

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Etoposide; PEGylated liposomes; Lyophilization; Lung cancer; Antitumor activity; Toxicity

摘要：Etoposide (VP16) is commonly used in the treatment of small cell lung cancer (SCLC) in clinical practice. However, severe adverse reactions such as bone marrow suppression toxicity limit its clinical application. Although several studies on VP16 liposomes were reported, no significant improvement in bone marrow suppression toxicity has been found, and there was a lack of validation of animal models for in vivo antitumor effects. Therefore, we attempted to develop a PEGylated liposomal formulation that effectively encapsulated VP16 (VP16-LPs) and evaluated its therapeutic effect and toxicity at the cellular level and in animal models. First, we optimized the preparation process of VP16-LPs using an orthogonal experimental design and further prepared them into freeze-dried powder to improve storage stability of the product. Results showed that VP16LPs freeze-dried powder exhibited good dispersibility and stability after redispersion. In addition, compared to marketed VP16 injection, VP16-LPs exhibited sustained drug release characteristics. At the cellular level, VP16LPs enhanced the cellular uptake of drugs and exhibited strong cytotoxic activity. In animal models, VP16-LPs could target and aggregate in tumors and exhibit a higher anti-tumor effect than VP16-injection after intravenous injection. Most importantly, hematological analysis results showed that VP16-LPs significantly alleviated the bone marrow suppression toxicity of drug. In summary, our study confirmed that PEGylated liposomes could enhance therapeutic efficacy and reduce toxicity of VP16, which demonstrated that VP16-LPs had enormous clinical application potential.

基金机构：Natural Science Foundation of Chongqing Municipal Bureau of Science and Technology [2022NSCQ-MSX0686]

基金资助正文：The authors gratefully acknowledge the financial support from the Natural Science Foundation of Chongqing Municipal Bureau of Science and Technology (2022NSCQ-MSX0686) .