Bone Regeneration Facilitated by Autologous Bioscaffold Material: Liquid Phase of Concentrated Growth Factor with Dental Follicle Stem Cell Loading

作者全名：Li, Zhentao; Wang, Di; Li, Jie; Liu, Hao; Nie, Li; Li, Conghua

作者地址：[Li, Zhentao; Wang, Di; Liu, Hao; Nie, Li; Li, Conghua] Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China; [Li, Jie] Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China

通信作者：Li, CH (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China.

来源：ACS BIOMATERIALS SCIENCE & ENGINEERING

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001203904300001

JCR分区：Q2

影响因子：5.4

年份：2024

卷号：10

期号：5

开始页：3173

结束页：3187

文献类型：Article

关键词：concentrated growth factor; dental follicle stem cells; bone regeneration; bioengineering

摘要：The application of bioengineering techniques for achieving bone regeneration in the oral environment is an increasingly prominent field. However, the clinical use of synthetic materials carries certain risks. The liquid phase of concentrated growth factor (LPCGF), as a biologically derived material, exhibits superior biocompatibility. In this study, LPCGF was employed as a tissue engineering scaffold, hosting dental follicle cells (DFCs) to facilitate bone regeneration. Both in vivo and in vitro experimental results demonstrate that this platform significantly enhances the expression of osteogenic markers in DFCs, such as alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and type I collagen (Col1a1). Simultaneously, it reduces the expression of inflammation-related genes, particularly interleukin-6 (IL-6) and interleukin-8 (IL-8), thereby alleviating the negative impact of the inflammatory microenvironment on DFCs. Further investigation into potential mechanisms reveals that this process is regulated over time by the WNT pathway. Our research results demonstrate that LPCGF, with its favorable physical characteristics, holds great potential as a scaffold. It can effectively carry DFCs, thereby providing an optimal initial environment for bone regeneration. Furthermore, LPCGF endeavors to closely mimic the mechanisms of bone healing post-trauma to facilitate bone formation. This offers new perspectives and insights into bone regeneration engineering.

基金机构：Natural Science Foundation of Chongqing Municipality [cstc2021jcyj-msxm0184]; Chongqing Natural Science Foundation General Project

基金资助正文：This work was supported by the Chongqing Natural Science Foundation General Project (grant number: cstc2021jcyj-msxm0184).