Unlocking the potential of β-1,3-xylooligosaccharides from <i>Caulerpa lentillifera</i>: structural characterization, antioxidative and anti-osteoarthritis applications

作者全名：Cai, Lixi; Zheng, Jinlin; Liu, Lixing; Chen, Xiaoping; Wang, Honglin

作者地址：[Cai, Lixi; Zheng, Jinlin; Liu, Lixing; Chen, Xiaoping] Putian Univ, Coll Basic Med, Putian 351100, Fujian, Peoples R China; [Wang, Honglin] Chongqing Med Univ, Dept Orthoped Surg, Dazu Hosp, Chongqing 402360, Peoples R China; [Cai, Lixi; Zheng, Jinlin; Liu, Lixing; Chen, Xiaoping] Putian Univ, Key Lab Translat Tumor Med Fujian Prov, Putian 351100, Fujian, Peoples R China

通信作者：Wang, HL (通讯作者)，Chongqing Med Univ, Dept Orthoped Surg, Dazu Hosp, Chongqing 402360, Peoples R China.

来源：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE

ESI学科分类：AGRICULTURAL SCIENCES

WOS号：WOS:001203988300001

JCR分区：Q1

影响因子：5.2

年份：2024

卷号：11

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：beta-1,3-xylan; beta-1,3-xylooligosaccharides; Antioxidant; Osteoarthritis; Enzymatic hydrolysis

摘要：Background beta-1,3-Xylooligosaccharides (XOS-3) extracted from Caulerpa lentillifera have shown potential antioxidative and anti-tumor properties, but research on their biological activities, particularly their anti-osteoarthritis effects, is still in early stages. Results XOS-3 were produced by eco-friendly enzymatic hydrolysis, displaying a semi-crystalline structure with predominant xylose, xylobiose, xylotriose, and xylotetraose components which were confirmed by IC, SEM, FT-IR, and XRD analysis. Compared with beta-1,4-xylooligosaccharides, XOS-3 had stronger antioxidant properties, and the scavenging rate of O-2(center dot-) and DPPH center dot radicals was 82.31% and 58.44% at 2.0 mg/mL respectively. In vitro studies, XOS-3 significantly improved cell viability in IL-1 beta-induced rat chondrocytes from 43.41 to 73.5% at 100 mu g/mL, demonstrating anti-inflammatory and cartilage-protective effects by modulating MMP13 and COL2A1 expression. Conclusions The study displayed the potential antioxidative and anti-osteoarthritis effects of XOS-3, offering new visuals for the development of marine polysaccharides in the treatment of osteoarthritis.

基金机构：Natural Science Foundation of Fujian Province of China

基金资助正文：Not applicable.