Caspase-8 dependent apoptosis contributes to dyskinesia caused by muscle defects and neurotoxicity in zebrafish exposed to zearalenone

作者全名：Wang, Yunpeng; Li, Shuaiting; Cheng, Zhi; Zhang, Ziyuan; Xu, Yu; Zhang, Huan; Xu, Ting; Chen, Jianqiang; Yin, Danyang; Yan, Wenhua; Huang, Huizhe

作者地址：[Wang, Yunpeng; Li, Shuaiting; Cheng, Zhi; Xu, Yu; Xu, Ting; Chen, Jianqiang; Yan, Wenhua; Huang, Huizhe] Chongqing Med Univ, Affiliated Hosp 2, Chongqing, Peoples R China; [Wang, Yunpeng; Cheng, Zhi; Zhang, Huan; Chen, Jianqiang] Chongqing Med Univ, Sch Basic Med Sci, Dept Cell Biol & Genet, Lab Dev Biol, Chongqing, Peoples R China; [Xu, Yu; Xu, Ting] Chongqing Coll Humanities, Sci & Technol, Chongqing, Peoples R China; [Yin, Danyang] Capital Med Univ, Beijing Friendship Hosp, Dept Pathol, Beijing, Peoples R China; [Zhang, Ziyuan] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders Chongqing, Res Inst,Dept Pediat,Minist Educ,Key Lab Child Dev, Box 136,3 Zhongshan RD, Chongqing 400014, Peoples R China; [Wang, Yunpeng; Zhang, Ziyuan] Chongqing Key Lab Pediat, Chongqing, Peoples R China

通信作者：Yan, WH; Huang, HZ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Chongqing, Peoples R China.

来源：FOOD AND CHEMICAL TOXICOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001204092200001

JCR分区：Q1

影响因子：3.9

年份：2024

卷号：186

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Zearalenone; Apoptosis; Muscle toxicity; Neurotoxicity; Dyskinesia

摘要：Zearalenone (ZEA), one of the usual mycotoxins, has been recognized in many areas and crops, posing a significant threat to the living organisms even to human beings. However, the mechanisms of locomotive defects remain unknown. Herein, zebrafish larvae was employed to investigate ZEA effects on developmental indexes, muscle and neural toxicity, apoptosis, transcriptome and motor behaviors of zebrafish larvae. Zebrafish larvae exposed to ZEA (0, 0.5, 1, 2 and 4 mu M) showed no change in survival rate, but the malformation rate of zebrafish larvae increased dramatically manifesting with severe body bending and accomplished with adverse effects on hatching rate and body length. Moreover, the larvae manifested with defective muscle and abnormal neural development, resulting in decreased swimming ability, which probably due to the abnormal overactivation of apoptosis. And this was confirmed by enriched caspase 8 -mediated apoptosis signaling pathway in the following transcriptome analysis. Meanwhile, there was a recovery in swimming behaviors in the larvae co -exposed in ZEA and caspase 8 inhibitor. These findings provide an important evidence for risk assessment and potential treatment target of ZEA exposure.

基金机构：National Natural Science Founda- tion of China [31771619]

基金资助正文：<BOLD>Funding</BOLD> This study was supported by the National Natural Science Founda- tion of China (Grant number 31771619 to Huizhe Huang) .