pGM-CSF as an adjuvant in DNA vaccination against SARS-CoV-2
作者全名：Liu, Chang; Xue, Ruo-Yi; Li, Guo-Cheng; Zhang, Yi; Wu, Wei-Yi; Liu, Jing-Yi; Feng, Rang; Jin, Zhe; Deng, Yan; Jin, Zi-Li; Cheng, Hao; Mao, Ling; Zou, Quan-Ming; Li, Hai-Bo
作者地址：[Liu, Chang; Xue, Ruo-Yi; Li, Guo-Cheng; Zhang, Yi; Wu, Wei-Yi; Liu, Jing-Yi; Feng, Rang; Jin, Zhe; Deng, Yan; Jin, Zi-Li; Cheng, Hao; Mao, Ling; Zou, Quan-Ming; Li, Hai-Bo] Third Mil Med Univ, Coll Pharm, Natl Engn Res Ctr Immunol Prod, Dept Microbiol & Biochem Pharm,Army Med Univ, Chongqing 400038, Peoples R China; [Liu, Chang] Chinese Peoples Liberat Army Unit 32265, Dept Pharm, Guangzhou 510310, Peoples R China; [Zou, Quan-Ming; Li, Hai-Bo] Third Mil Med Univ, Army Med Univ, Coll Pharm, Chongqing 400038, Peoples R China
通信作者：Zou, QM; Li, HB (通讯作者)，Third Mil Med Univ, Army Med Univ, Coll Pharm, Chongqing 400038, Peoples R China.
来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ESI学科分类：BIOLOGY & BIOCHEMISTRY
WOS号：WOS:001205035600001
JCR分区：Q1
影响因子：7.7
年份：2024
卷号：264
期号：
开始页：
结束页：
文献类型：Article
关键词：SARS-CoV-2; DNA vaccine; Adjuvant; GM-CSF
摘要：The emergence of SARS-CoV-2 presents a significant global public health dilemma. Vaccination has long been recognized as the most effective means of preventing the spread of infectious diseases. DNA vaccines have attracted attention due to their safety profile, cost-effectiveness, and ease of production. This study aims to assess the efficacy of plasmid-encoding GM-CSF (pGM-CSF) as an adjuvant to augment the specific humoral and cellular immune response elicited by DNA vaccines based on the receptor-binding domain (RBD) antigen. Compared to the use of plasmid-encoded RBD (pRBD) alone, mice that were immunized with a combination of pRBD and pGM-CSF exhibited significantly elevated levels of RBD-specific antibody titers in serum, BALF, and nasal wash. Furthermore, these mice generated more potent neutralization antibodies against both the wild-type and Omicron pseudovirus, as well as the ancestral virus. In addition, pGM-CSF enhanced pRBD-induced CD4+ and CD8+ T cell responses and promoted central memory T cells storage in the spleen. At the same time, tissue-resident memory T (Trm) cells in the lung also increased significantly, and higher levels of specific responses were maintained 60 days post the final immunization. pGM-CSF may play an adjuvant role by promoting antigen expression, immune cells recruitment and GC B cell responses. In conclusion, pGM-CSF may be an effective adjuvant candidate for the DNA vaccines against SARS-CoV-2.
基金机构：Chongqing Natural Science Foundation [cstc2020jcyj-zdxmX0027, cstc2020jcyj-msxmX0353]; Chinese National Natural Science Foundation [32270988]; National Key Research and Development Program of China [2021YFC2302603]
基金资助正文：We are grateful to Dr. Yao Yanfeng's group from the Wuhan Institute of Virology, Chinese Academy of Sciences, for help with the neutralizing antibody titer test. This work was supported by Chongqing Natural Science Foundation (No. cstc2020jcyj-zdxmX0027, cstc2020jcyj-msxmX0353), Chinese National Natural Science Foundation Project (No. 32270988) and National Key Research and Development Program of China (2021YFC2302603).
