Transcriptome and single-cell analysis reveal disulfidptosis-related modification patterns of tumor microenvironment and prognosis in osteosarcoma
作者全名：Wang, Linbang; Liu, Yu; Tai, Jiaojiao; Dou, Xinyu; Yang, Hongjuan; Li, Qiaochu; Liu, Jingkun; Yan, Ziqiang; Liu, Xiaoguang
作者地址：[Wang, Linbang; Liu, Yu; Dou, Xinyu; Liu, Xiaoguang] Peking Univ Third Hosp, Dept Orthopaed, Beijing, Peoples R China; [Tai, Jiaojiao; Liu, Jingkun; Yan, Ziqiang] Xi An Jiao Tong Univ, Honghui Hosp, Dept Orthoped, 555,Youyi Rd, Xian 710054, Shaanxi, Peoples R China; [Yang, Hongjuan] Xian Med Univ, Sch Foreign Studies, Xian 710054, Shaanxi, Peoples R China; [Li, Qiaochu] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped Surg, Chongqing 400016, Peoples R China
通信作者：Liu, XG (通讯作者)，Peking Univ Third Hosp, Dept Orthopaed, Beijing, Peoples R China.; Liu, JK; Yan, ZQ (通讯作者)，Xi An Jiao Tong Univ, Honghui Hosp, Dept Orthoped, 555,Youyi Rd, Xian 710054, Shaanxi, Peoples R China.
来源：SCIENTIFIC REPORTS
ESI学科分类：Multidisciplinary
WOS号：WOS:001206473300028
JCR分区：Q1
影响因子：3.8
年份：2024
卷号：14
期号：1
开始页：
结束页：
文献类型：Article
关键词：Disulfidptosis; Osteosarcoma; Single-cell analysis; ACTB; HMGB1
摘要：Osteosarcoma (OS) is the most common malignant bone tumor with high pathological heterogeneity. Our study aimed to investigate disulfidptosis-related modification patterns in OS and their relationship with survival outcomes in patients with OS. We analyzed the single-cell-level expression profiles of disulfidptosis-related genes (DSRGs) in both OS microenvironment and OS subclusters, and HMGB1 was found to be crucial for intercellular regulation of OS disulfidptosis. Next, we explored the molecular clusters of OS based on DSRGs and related immune cell infiltration using transcriptome data. Subsequently, the hub genes of disulfidptosis in OS were screened by applying multiple machine models. In vitro and patient experiments validated our results. Three main disulfidptosis-related molecular clusters were defined in OS, and immune infiltration analysis suggested high immune heterogeneity between distinct clusters. The in vitro experiment confirmed decreased cell viability of OS after ACTB silencing and higher expression of ACTB in patients with lower immune scores. Our study systematically revealed the underlying relationship between disulfidptosis and OS at the single-cell level, identified disulfidptosis-related subtypes, and revealed the potential role of ACTB expression in OS disulfidptosis.
基金机构：National Natural Science Foundation of China [81972103]; Pharmaceutical Innovative Varieties Research and Development and Industry Support Platform Capacity Construction Project [Z191100007619023]; Science and Technology Commission of Beijing Municipality,
基金资助正文：National Natural Science Foundation of China (Approval number: 81972103). Science and Technology Commission of Beijing Municipality, Pharmaceutical Innovative Varieties Research and Development and Industry Support Platform Capacity Construction Project (Approval number: Z191100007619023).
