Potential AhR-independent mechanisms of 2,3,7,8-Tetrachlorodibenzo-p-dioxin inhibition of human glioblastoma A172 cells migration

作者全名：Liu, Yiyun; Zhu, Ruihong; Xu, Tuan; Chen, Yangsheng; Ding, Yuan; Zuo, Sijia; Xu, Li; Xie, Heidi Qunhui; Zhao, Bin

作者地址：[Liu, Yiyun; Zhu, Ruihong; Xu, Tuan; Chen, Yangsheng; Xu, Li; Xie, Heidi Qunhui; Zhao, Bin] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing, Peoples R China; [Zhu, Ruihong; Xu, Tuan; Chen, Yangsheng; Xu, Li; Xie, Heidi Qunhui; Zhao, Bin] Univ Chinese Acad Sci, Beijing, Peoples R China; [Liu, Yiyun; Ding, Yuan; Zuo, Sijia] Chongqing Med Univ, Sch Publ Hlth, Chongqing, Peoples R China; [Xie, Heidi Qunhui] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China

通信作者：Xie, HQ (通讯作者)，Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China.

来源：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ESI学科分类：ENVIRONMENT/ECOLOGY

WOS号：WOS:001206652800001

JCR分区：Q1

影响因子：6.2

年份：2024

卷号：273

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：TCDD; Glioblastoma; Aryl hydrocarbon receptor; Migration

摘要：The toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is generally believed to be mediated by aryl hydrocarbon receptor (AhR), but some evidence suggests that the effects of TCDD can also be produced through AhR-independent mechanisms. In previous experiments, we found that mainly AhR-dependent mechanism was involved in the migration inhibition of glioblastoma U87 cells by TCDD. Due to the heterogeneity of glioblastomas, not all tumor cells have significant AhR expression. The effects and mechanisms of TCDD on the migration of glioblastomas with low AhR expression are still unclear. We employed a glioblastoma cell line A172 with low AhR expression as a model, using wound healing and Transwell (R) assay to detect the effect of TCDD on cell migration. We found that TCDD can inhibit the migration of A172 cells without activating AhR signaling pathway. Further, after being pre-treated with AhR antagonist CH223191, the inhibition of TCDD on A172 cells migration was not changed, indicating that the effect of TCDD on A172 cells is not dependent on AhR activation. By transcriptome sequencing analysis, we propose dysregulation of the expression of certain migration-related genes, such as IL6, IL1B, CXCL8, FOS, SYK, and PTGS2 involved in cytokines, MAPK, NF-kappa B, and IL-17 signaling pathways, as potential AhR-independent mechanisms that mediate the inhibition of TCDD migration in A172 cells.

基金机构：National Key Research and Development Program of China [2018YFA0901102]; Chongqing Postdoctoral Science Foundation [2022TQ0394, CSTB2022NSCQ- BHX0641]; Chongqing Special Funding for Postdoctoral Research Projects [2022CQBSHTB1006]

基金资助正文：This work was supported by the National Key Research and Development Program of China (2018YFA0901102) , China and Chongqing Postdoctoral Science Foundation (2022TQ0394, CSTB2022NSCQ- BHX0641) , Chongqing Special Funding for Postdoctoral Research Projects (2022CQBSHTB1006) .