Self-enhanced nanohydrogel electrochemiluminescence biosensor based on CRISPR/Cas12a and gold platinum nanoparticles modification for high-sensitivity detection of<i> Burkholderia</i><i> pseudomallei</i>

作者全名：Wang, Yuexin; Shen, Bo; Luo, Nini; Li, Cai; Wu, Haiping; Wang, Yanshuang; Tian, Shen; Li, Xuemiao; Liu, Rui; Li, Xinmin; Chen, Junman; Cheng, Wei; Ding, Shijia; Chen, Rui; Xiao, Meifang; Xia, Qianfeng

作者地址：[Wang, Yuexin; Luo, Nini; Li, Cai; Wang, Yanshuang; Tian, Shen; Li, Xuemiao; Liu, Rui; Xia, Qianfeng] Hainan Med Univ, Affiliated Hosp 2, Sch Trop Med, NHC Key Lab Control Trop Dis,Dept Trop Dis, Haikou 571199, Hainan, Peoples R China; [Wang, Yuexin; Xiao, Meifang] Hainan Women & Childrens Med Ctr, Haikou 571199, Hainan, Peoples R China; [Shen, Bo; Wu, Haiping; Li, Xinmin; Chen, Junman; Ding, Shijia; Chen, Rui] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Cheng, Wei] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China

通信作者：Xia, QF (通讯作者)，Hainan Med Univ, Affiliated Hosp 2, Sch Trop Med, NHC Key Lab Control Trop Dis,Dept Trop Dis, Haikou 571199, Hainan, Peoples R China.; Xiao, MF (通讯作者)，Hainan Women & Childrens Med Ctr, Haikou 571199, Hainan, Peoples R China.; Chen, R (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China.

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:001206697900001

JCR分区：Q1

影响因子：13.3

年份：2024

卷号：486

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Burkholderia pseudomallei; Self-enhanced Au@PEI-ABEI@Pt porous; hydrogel; CRISPR/Cas12a; Electrochemiluminescence

摘要：The simple, rapid, and accurate detection of highly lethal melioidosis is crucial for early clinical diagnosis and improving cure rates. Currently, due to time consumption, low sensitivity and detection rate the existing clinical detection methods cannot satisfy the needs of clinical diagnosis. Herein, a novel self-enhanced porous hydrogel material (Au@PEI-ABEI@Pt) for ultrasensitive ECL strategy of detection B. pseudomallei was report. The novel porous hydrogel material composed of PEI-ABEI porous hydrogel, gold nanoparticles (AuNP) and platinum nanoparticles (PtNP) have large specific surface area and porous structure, which not only fix more ABEI to realize self-enhanced ECL signal amplification, but also facilitate ion diffusion and efficient utilization of catalytic materials, realizing rapid electron transfer and zero -distance catalysis, significantly improving the initial signal of ECL sensor. Moreover, the most attractive aspect is that Au@PEI-ABEI@Pt hydrogels with good biocompatibility can achieve widespread application of CRISPR/Cas12a in solid -phase carriers without affecting the sensitivity, specificity and shearing activity of CRISPR/Cas12a. After coupling with the ECL system and CRISPR/Cas12a signal amplification strategy, the Au@PEI-ABEI@Pt can achieve an ultrasensitive ECL assay of B. pseudomallei with the LOD of 5 CFU mL-1 in complex samples, with high specificity and stability to effectively classify B. pseudomallei and other Gram -negative bacteria. This study shows that the developed porous hydrogel materials not only serve as an excellent ECL signal reporter to significantly improve the detection sensitivity of ECL biosensors, but also provide a new approach for the wide application of CRISPR/Cas systems in solid -phase carriers.

基金机构：Major Science and Technology Program of Hainan Province [ZDKJ202003, ZDKJ2021036]; National Natural Science Foundation of China [81960002, 82370018]; Science and Technology Plan of Hainan Province (Hainan Province Clinical Medical Center); Natural Science Foundation of Hainan Provincial High Level Talent Project [823RC598]; Natural Science Foundation Project of Chongqing [cstc2021jcyj-msxmX0325]

基金资助正文：<STRONG> </STRONG>This study was supported by the Major Science and Technology Program of Hainan Province (ZDKJ202003, ZDKJ2021036), the National Natural Science Foundation of China (81960002, 82370018), Science and Technology Plan of Hainan Province (Hainan Province Clinical Medical Center) , The Natural Science Foundation of Hainan Provincial High Level Talent Project (823RC598) , and the Natural Science Foundation Project of Chongqing (cstc2021jcyj-msxmX0325) .