Effects of Low-Intensity Pulsed Ultrasound on the Regulation of Free Fatty Acid Release in 3T3-L1 Cells

作者全名："Wu, Liu; Xiao, Xinfang; Deng, Juan; Zhou, Yiqing; Li, Junfen; He, Sicheng; Wang, Yan"

作者地址："[Wu, Liu; Xiao, Xinfang; Deng, Juan; Zhou, Yiqing; Li, Junfen; He, Sicheng; Wang, Yan] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing Key Lab Biomed Engn, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China"

通信作者："Wang, Y (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing Key Lab Biomed Engn, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：JOURNAL OF ULTRASOUND IN MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001206766000001

JCR分区：Q2

影响因子：2.3

年份：2024

卷号：43

期号：8

开始页：1449

结束页：1460

文献类型：Article

关键词：free fatty acids; lipotoxicity; low-intensity pulsed ultrasound; tumor necrosis factor-alpha

摘要："Objectives-To investigate the effects of low-intensity pulsed ultrasound (LIPUS) on the proliferation, differentiation, and tumor necrosis factor-alpha (TNF-alpha)-induced lipolysis of 3T3-L1 cells, and to explore the feasibility of regulating the release of free fatty acids (FFA) to prevent lipotoxicity. Methods-Different intensities (30, 60, 90, and 120 mW/cm(2)) of LIPUS were applied to 3T3-L1 preadipocytes for different durations (5, 10, 15, 20, 25, and 30 minutes). Appropriate parameters for subsequent experiments were selected by assessing cell viability. The effect of LIPUS on the proliferation and differentiation of 3T3-L1 cells was evaluated by microscope observation, flow cytometry, and lipid content determination. After treated with LIPUS and TNF-alpha (50 ng/mL), the degree of lipolysis was assessed by measuring the extracellular FFA content. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression of relevant genes. Results-Different parameters of LIPUS significantly enhance the viability of 3T3-L1 cells (P < .05), with 20 minutes and 30 mW/cm(2) as the most suitable settings. After LIPUS treatment, 3T3-L1 cell proliferation accelerated, apoptosis rate and G1 phase cell proportion decreased, the content of lipid droplets and TG was increased in differentiated cells, while FFA release decreased (P < .05). The expression of PCNA, PPAR gamma, C/EBP alpha, Perilipin A mRNA increased, and the expression of TNF-alpha, ATGL, HSL mRNA decreased (P < .05). Conclusions-LIPUS could promote the proliferation and differentiation of 3T3-L1 cells and inhibit TNF-alpha-induced lipolysis, indicating its potential as a therapy for mitigating lipotoxicity caused by decompensated adipocytes."

基金机构：Chongqing Medical University 2022 Future Medical Youth Innovation Team Development Support Program Project

基金资助正文：No Statement Available