Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+T Cells During Tumorigenesis

作者全名："Zhang, Yan; Su, Xingxing; Wang, Lisha; Yue, Zhengliang; Liu, Qiao; Ran, Ling; Lei, Shun; Hu, Jianjun; Xu, Lifan; Ye, Lilin; Ji, Ping; Li, Guimei; Huang, Qizhao; Wen, Shuqiong"

作者地址："[Zhang, Yan; Li, Guimei] Qingdao Agr Univ, Coll Vet Med, Qingdao, Peoples R China; [Su, Xingxing; Wang, Lisha; Yue, Zhengliang; Ran, Ling; Lei, Shun; Hu, Jianjun; Xu, Lifan; Ye, Lilin] Third Mil Med Univ, Inst Immunol, Chongqing, Peoples R China; [Liu, Qiao] Third Mil Med Univ, Xinqiao Hosp, Inst Canc, Chongqing, Peoples R China; [Ji, Ping; Wen, Shuqiong] Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China; [Huang, Qizhao] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China"

通信作者："Li, GM (通讯作者)，Qingdao Agr Univ, Coll Vet Med, Qingdao, Peoples R China.; Wen, SQ (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China.; Huang, QZ (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China."

来源：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001207641700025

JCR分区：Q3

影响因子：1.2

年份：2024

卷号：　

期号：203

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Tumor antigen -specific CD8+ T cells from draining lymph nodes gain an accumulating importance in mounting anti -tumor immune response during tumorigenesis. However, in many cases, cancer cells form metastatic loci in lymph nodes before further metastasizing to distant organs. To what extent the local and systematic CD8+ T cell responses were influenced by LN metastasis remains obscure. To this end, we set up a murine LN metastasis model combined with a B16F10-GP melanoma cell line expressing the surrogate neoantigen derived from lymphocytic choriomeningitis virus (LCMV), glycoprotein (GP), and P14 transgenic mice harboring T cell receptors (TCRs) specific to GP -derived peptide GP33-41 presented by the class I major histocompatibility complex (MHC) molecule H-2Db. This protocol enables the study of antigen -specific CD8+ T cell responses during LN metastasis. In this protocol, C57BL/6J mice were subcutaneously implanted with B16F10-GP cells, followed by adoptive transfer with naive P14 cells. When the subcutaneous tumor grew to approximately 5 mm in diameter, the primary tumor was excised, and B16F10-GP cells were directly injected into the tumor draining lymph node (TdLN). Then, the dynamics of CD8+ T cells were monitored during the process of LN metastasis. Collectively, this model has provided an approach to precisely investigate the antigen -specific CD8+ T cell immune responses during LN metastasis."

基金机构：National Science Foundation for Outstanding Young Scholars of China [82122028]; National Natural Science Foundation of China [82173094]; Natural Science Foundation of Chong Qing [2023NSCQ-BHX0087]

基金资助正文："This work was supported by the National Science Foundation for Outstanding Young Scholars of China (No. 82122028 to LX) , the National Natural Science Foundation of China (No. 82173094 to LX) , Natural Science Foundation of Chong Qing (No. 2023NSCQ-BHX0087 to SW) ."