Characterization of cells in blood evoked from periapical tissues in immature teeth with pulp necrosis and their potential for autologous cell therapy in Regenerative Endodontics

作者全名：Zheng, Chengxiang; Jiang, Peiru; Hu, Shan; Tang, Yin; Dou, Lei

作者地址：[Zheng, Chengxiang; Jiang, Peiru; Hu, Shan; Dou, Lei] Chongqing Med Univ, Affiliated Stomatol Hosp, 426 Song Shi Bei Rd, Chongqing 401147, Peoples R China; [Zheng, Chengxiang; Jiang, Peiru; Hu, Shan; Dou, Lei] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Zheng, Chengxiang; Jiang, Peiru; Hu, Shan; Dou, Lei] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Tang, Yin] Western Univ Hlth Sci, Sch Dent Med, Pomona, CA USA

通信作者：Dou, L (通讯作者)，Chongqing Med Univ, Affiliated Stomatol Hosp, 426 Song Shi Bei Rd, Chongqing 401147, Peoples R China.

来源：ARCHIVES OF ORAL BIOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001209282600001

JCR分区：Q2

影响因子：2.2

年份：2024

卷号：162

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Apical periodontitis; Autologous; Immature tooth; Regenerative Endodontics; Stem cell

摘要：Objective: The objectives of this study were to isolate, characterize progenitor cells from blood in the root canals of necrotic immature permanent teeth evoked from periapical tissues and evaluate the applicable potential of these isolated cells in Regenerative Endodontics. Design: Ten necrotic immature permanent teeth from seven patients were included. Evoked bleeding from periapical tissues was induced after chemical instrumentation of the root canals. Cells were isolated from the canal blood and evaluated for cell surface marker expression, multilineage differentiation potential, proliferation ability, and target protein expression. Cell sheets formed from these cells were transferred into human root segments, and then transplanted into nude mice. Histological examination was performed after eight weeks. Data analysis was conducted using one-way ANOVA followed by Tukey's post-hoc comparison, considering p < 0.05 as statistically significant. Results: The isolated cells exhibited characteristics typical of fibroblastic cells with colony-forming efficiency, and displayed Ki67 positivity and robust proliferation. Flow cytometry data demonstrated that at passage 3, these cells were positive for CD73, CD90, CD105, CD146, and negative for CD34 and CD45. Vimentin expression indicated a mesenchymal origin. Under differentiation media specific differentiation media, the cells demonstrated osteogenic, adipogenic, and chondrogenic differentiation potential. Subcutaneous root canals with cell sheets of isolated cells in nude mice showed the formation of pulp-like tissues. Conclusions: This study confirmed the presence of progenitor cells in root canals following evoked bleeding from periapical tissues of necrotic immature teeth. Isolated cells exhibited similar immunophenotype and regenerative potential with dental mesenchymal stromal cells in regenerative endodontic therapy.

基金机构：Chongqing Municipal Health Commis- sion [2023GDRC012]; Chongqing Municipal Education Com- mission [KJQN202300469]; National Natural Science Foundation of China [81800958]

基金资助正文：This study was sponsored by Chongqing Municipal Health Commis- sion (grant no. 2023GDRC012) , Chongqing Municipal Education Com- mission (grant no. KJQN202300469) and National Natural Science Foundation of China (grant no. 81800958) . The authors deny any con- flicts of interest related to this study.