A novel entropy-driven dual-output mode integrated with DNAzyme for enhanced microRNA detection

作者全名：Zhang, Jianhong; Bai, Dan; Xie, Guoming; Xie, Yaxing; Lin, Yu; Hou, Yulei; Yu, Ying; Zhang, Yaoyi; Zhao, Rong; Wang, Zhongzhong; Wang, Luojia; Chen, Hui

作者地址：[Zhang, Jianhong; Lin, Yu; Hou, Yulei; Yu, Ying; Chen, Hui] Chongqing Med Univ, Affiliated Hosp 1, Clin Labs, 1 You Yi Rd, Chongqing 400016, Peoples R China; [Bai, Dan; Xie, Guoming; Xie, Yaxing; Zhang, Yaoyi; Zhao, Rong; Wang, Zhongzhong; Wang, Luojia] Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost, Chinese Minist Educ, Chongqing 400016, Peoples R China

通信作者：Chen, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Clin Labs, 1 You Yi Rd, Chongqing 400016, Peoples R China.

来源：TALANTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001214708800001

JCR分区：Q1

影响因子：5.6

年份：2024

卷号：275

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Entropy -driven catalysis; Dual -output mode; DNAzyme; Signal amplification; MicroRNA detection

摘要：Accurate microRNA (miRNA) detection is pivotal in the diagnosis and monitoring of cancer. Entropy-driven catalysis (EDC) has attracted widespread attention as an enzyme-free, isothermal technique for miRNA detection owing to its inherent simplicity and reliability. However, conventional EDC is a single-output mode, limiting the efficiency of signal amplification. In this study, a novel EDC dual-output mode was employed in conjunction with DNAzyme, resulting in the development of an EDC dual-end DNAzyme (EDC-DED) approach for highly sensitive miRNA detection. In this system, miRNA-21 initiated the EDC reaction, producing a large amount of catalytically active dual-end Mg2+-dependent DNAzyme. The DNAzyme further cleaved the reporter cyclically, generating a notably amplified fluorescence signal. The proposed method achieved a low detection limit of 2 pM. Compared with the traditional EDC single-end DNAzyme (EDC-SED) strategy, the present method exhibited superior amplification efficiency, enhancing detection sensitivity by approximately 46.5-fold. Furthermore, this platform demonstrated ideal specificity, satisfactory reproducibility and acceptable detection capabilities in clinical serum samples. Therefore, the straightforward and convenient strategy is a potential tool for miRNA analysis, which may provide a new perspective for biological analysis and clinical application.

基金机构：Natural Science Foundation of Chongqing Municipality, China [CSTB2022NSCQ-MSX0101, CSTB2023NSCQ-MSX0147]; National Natural Science Foundation of China [81972011]

基金资助正文：<B>Acknowledgments</B> This research work was financially supported by the Natural Science Foundation of Chongqing Municipality, China (No. CSTB2022NSCQ-MSX0101 and CSTB2023NSCQ-MSX0147) , and the National Natural Science Foundation of China (No. 81972011) .