Running exercise improves astrocyte loss, morphological complexity and astrocyte-contacted synapses in the hippocampus of CUS-induced depression model mice
作者全名：Li, Yue; Luo, Yanmin; Zhu, Peilin; Liang, Xin; Li, Jing; Dou, Xiaoyun; Liu, Li; Qin, Lu; Zhou, Mei; Deng, Yuhui; Jiang, Lin; Wang, Shun; Yang, Wenyu; Tang, Jing; Tang, Yong
作者地址：[Li, Yue; Zhu, Peilin; Li, Jing; Liu, Li; Qin, Lu; Deng, Yuhui; Wang, Shun; Tang, Jing; Tang, Yong] Chongqing Med Univ, Sch Basic Med Sci, Dept Histol & Embryol, Chongqing 400016, Peoples R China; [Li, Yue; Luo, Yanmin; Zhu, Peilin; Liang, Xin; Li, Jing; Liu, Li; Qin, Lu; Zhou, Mei; Deng, Yuhui; Jiang, Lin; Wang, Shun; Yang, Wenyu; Tang, Jing; Tang, Yong] Chongqing Med Univ, Sch Basic Med Sci, Lab Stem Cells & Tissue Engn, Chongqing 400016, Peoples R China; [Luo, Yanmin; Zhou, Mei; Yang, Wenyu] Chongqing Med Univ, Sch Basic Med Sci, Dept Physiol, Chongqing 400016, Peoples R China; [Liang, Xin] Chongqing Med Univ, Sch Basic Med Sci, Dept Pathol, Chongqing 400016, Peoples R China; [Dou, Xiaoyun] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China; [Jiang, Lin] Chongqing Med Univ, Lab Teaching & Management Ctr, Chongqing 400016, Peoples R China; [Tang, Jing; Tang, Yong] Chongqing Med Univ, Fac Basic Med Sci, Dept Histol & Embryol, Chongqing 400016, Peoples R China
通信作者：Tang, J; Tang, Y (通讯作者)，Chongqing Med Univ, Fac Basic Med Sci, Dept Histol & Embryol, Chongqing 400016, Peoples R China.
来源：PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
ESI学科分类：NEUROSCIENCE & BEHAVIOR
WOS号：WOS:001215849500001
JCR分区：Q1
影响因子：3.3
年份：2024
卷号：239
期号：
开始页：
结束页：
文献类型：Article
关键词：Running exercise; Depression; Hippocampus; Astrocyte; Synapse
摘要：Although the antidepressant effects of running exercise have been widely reported, further research is still needed to determine the structural bases for these effects. Astrocyte processes physically contact many synapses and directly regulate the numbers of synapses, but it remains unclear whether running exercise can modulate astrocyte morphological complexity and astrocyte-contacted synapses in the hippocampus of the mice with depressive -like behavior. Male C57BL/6 J mice underwent four weeks of running exercise after four weeks of chronic unpredictable stress (CUS). The sucrose preference test (SPT), tail suspension test (TST) and forced swim test (FST) were used to assess anhedonia in mice. Western blotting was used to measure the expression of astrocyte- and synapse -related proteins. Immunofluorescence and 3D reconstruction were used to quantify the density and morphology of astrocytes, and astrocyte-contacted synapses in each hippocampal subregion. Four weeks of running exercise alleviated depressive -like symptoms in mice. The expression of astrocyte- and synapserelated proteins in the hippocampus; astrocyte process lengths, process numbers, and dendritic arborization; and the number of astrocyte-contacted PSD95 positive synapses in the CA2 -3 and DG regions were significantly decreased in the mice with depressive -like behavior, and running exercise successfully reserved these changes. Running exercise improved the decreases in astrocyte morphological complexity and astrocyte-contacted PSD95 positive synapses in the CA2 -3 and DG regions of the mice with depressive -like behavior, suggesting that the physical interactions between astrocytes and synapses can be increased by running exercise, which might be an important structural basis for the antidepressant effects of running exercise.
基金机构：National Natural Science Foundation of China [82171522, 82001436]; Natural Science Foundation of Chongqing, P. R. China [cstc2021jcyj-msxmX0110]
基金资助正文：This work was supported by the National Natural Science Foundation of China (82171522, 82001436) ; and Natural Science Foundation of Chongqing, P. R. China (cstc2021jcyj-msxmX0110) .
