"Multifunctional Composite Scaffold with Nanosilver, Graphene Oxide, and Macrophage Membrane Vesicles for Sequential Treatment of Infected Bone Defects"

作者全名："Sun, Mingjie; Lu, Yang; Zhang, Hongrui; Jiang, Weiqian; Wang, Wenzhao; Huang, Xiao; Zhang, Shichun; Xiang, Dulei; Tang, Boyu; Chen, Yu; Chen, Tingmei; Lian, Chengjie; Zhang, Jian"

作者地址："[Sun, Mingjie; Zhang, Hongrui; Huang, Xiao; Xiang, Dulei; Tang, Boyu; Chen, Yu; Lian, Chengjie; Zhang, Jian] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400016, Peoples R China; [Lu, Yang] Chinese Acad Sci, Ctr Human Tissues & Organs Degenerat, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China; [Jiang, Weiqian; Zhang, Shichun] Chongqing Med Univ, Affiliated Hosp 2, Dept Orthoped, Chongqing 401336, Peoples R China; [Wang, Wenzhao] Shandong Univ, Qilu Hosp, Cheeloo Coll Med, Dept Orthoped, Jinan 250012, Peoples R China; [Chen, Tingmei] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Lian, CJ; Zhang, J (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400016, Peoples R China."

来源：ADVANCED HEALTHCARE MATERIALS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001216440900001

JCR分区：Q1

影响因子：10

年份：2024

卷号：13

期号：20

开始页：　

结束页：　

文献类型：Article

关键词：graphene oxide; immunoregulation; infected bone defect; membrane vesicles; silver nanoparticles

摘要："The management of infected bone defects poses a significant clinical challenge, and current treatment modalities exhibit various limitations. This study focuses on the development of a multifunctional composite scaffold comprising nanohydroxyapatite/polyethyleneglycol diacrylate matrix, silver nanoparticles, graphene oxide (GO), sodium alginate, and M2-type macrophage membrane vesicles (MVs) to enhance the healing of infected bone defects. The composite scaffold demonstrates several key features: first, it releases sufficient quantities of silver ions to effectively eliminate bacteria; second, the controlled release of MVs leads to a notable increase in M2-type macrophages, thereby significantly mitigating the inflammatory response. Additionally, GO acts synergistically with nanohydroxyapatite to enhance osteoinductive activity, thereby fostering bone regeneration. Through meticulous in vitro and in vivo investigations, the composite scaffold exhibits broad-spectrum antimicrobial effects, robust immunomodulatory capabilities, and enhanced osteoinductive activity. This multifaceted composite scaffold presents a promising approach for the sequential treatment of infected bone defects, addressing the antimicrobial, immunomodulatory, and osteogenic aspects. This study introduces innovative perspectives and offers new and effective treatment alternatives for managing infected bone defects. This study successfully fabricates a multifunctional integrated composite scaffold using 3D printing technology, which encompasses nanosilver, graphene oxide, and macrophage membrane vesicles. This composite scaffold demonstrates excellent antibacterial, immunomodulatory, and osteogenic properties, and exhibits favorable biocompatibility in vivo. This provides a potential strategy for the sequential treatment of infected bone defects. image"

基金机构：National Natural Science Foundation of China; Chongqing Natural Science Foundation [CSTB2023NSCQ-LZX0018]; Youth Talent Support Program of the First Affiliated Hospital of Chongqing Medical University [BJRC2021-02]; Science and Technology Research Project of Chongqing Education Commission [KJQN202200404]; [82102610]

基金资助正文："This work was supported by National Natural Science Foundation of China (3227090327), the National Natural Science Foundation of China (82102610), Chongqing Natural Science Foundation Key Project (CSTB2023NSCQ-LZX0018), Youth Talent Support Program of the First Affiliated Hospital of Chongqing Medical University (BJRC2021-02), and the Science and Technology Research Project of Chongqing Education Commission (KJQN202200404)."