Proximity hybridization based "turn-on" DNA tweezers for accurate and enzyme-free small extracellular vesicle analysis

作者全名：Wu, Jinlin; Mei, Xi; Zhan, Xiaoqin; Liu, Fang; Liu, Dongfang

作者地址：[Wu, Jinlin; Mei, Xi; Liu, Dongfang] Chongqing Med Univ, Affiliated Hosp 2, Dept Endocrinol, 74 Linjiang Rd, Chongqing 400010, Peoples R China; [Wu, Jinlin; Liu, Fang] Chongqing Hosp Tradit Chinese Med, Dept Endocrinol, Chongqing 400021, Peoples R China; [Zhan, Xiaoqin] Chongqing Hosp Tradit Chinese Med, Dept Clin Lab, Chongqing 400021, Peoples R China; [Zhan, Xiaoqin] Chongqing Hosp Tradit Chinese Med, Chongqing Key Lab Sichuan Chongqing Coconstruct Di, Chongqing 400021, Peoples R China; [Mei, Xi] First Affiliated Hosp, Affiliated Hosp 1, Dept Endocrinol & Metab, Chengdu 610500, Sichuan, Peoples R China

通信作者：Liu, DF (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Endocrinol, 74 Linjiang Rd, Chongqing 400010, Peoples R China.

来源：ANALYTICAL METHODS

ESI学科分类：CHEMISTRY

WOS号：WOS:001217096100001

JCR分区：Q1

影响因子：2.7

年份：2024

卷号：16

期号：19

开始页：3118

结束页：3124

文献类型：Article

关键词：　

摘要：Small extracellular vesicles (sEVs) are a type of extracellular vesicle that carries many types of molecular information. The identification of sEVs is essential for the non-invasive detection and treatment of illnesses. Hence, there is a significant need for the development of simple, sensitive, and precise methods for sEV detection. Herein, a DNA tweezers-based assay utilizing a "turn-on" mechanism and proximity ligation was suggested for the efficient and rapid detection of sEVs through amplified fluorescence. The target facilitates the proximity combination of the C1 probe and C2 probe, resulting in the formation of a complete extended sequence. The elongated sequence can cyclically initiate the hairpin probe (HP), leading to the activation of DNA tweezers. An excellent linear correlation was achieved, with a limit of detection of 57 particles per mu L. Furthermore, it has been effectively employed to analyze sEVs under intricate experimental conditions, demonstrating a promising and pragmatic prospect for future applications. Given that the identification of sEVs was successfully accomplished using a single-step method that exhibited exceptional sensitivity and strong resistance to interference, the proposed technique has the potential to provide a beneficial platform for accurate recognition of sEVs and early detection of diseases. Small extracellular vesicles (sEVs) are a type of extracellular vesicle that carries many types of molecular information.

基金机构：Chongqing Medical Scientific Research Project (Joint of Chongqing Health Commission and Science and Technology Bureau) [2022MSXM158]

基金资助正文：This work was supported by the Chongqing Medical Scientific Research Project (Joint of Chongqing Health Commission and Science and Technology Bureau) (project number: 2022MSXM158).