CircPRELID2 functions as a promoter of renal cell carcinoma through the miR-22-3p/ETV1 cascade

作者全名：Lin, Xi; Zhi, Yi

作者地址：[Lin, Xi; Zhi, Yi] Chongqing Med Univ, Affiliated Hosp 3, Dept Urol, 1 Shuanghu Branch Rd,Huixing St, Chongqing 401120, Peoples R China

通信作者：Zhi, Y (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Dept Urol, 1 Shuanghu Branch Rd,Huixing St, Chongqing 401120, Peoples R China.

来源：BMC UROLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001217964400001

JCR分区：Q3

影响因子：1.7

年份：2024

卷号：24

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：RCC; circPRELID2; miR-22-3p; ETV1 expression; Malignant progression

摘要：Background Emerging evidence has indicated that a number of circular RNAs (circRNAs) participate in renal cell carcinoma (RCC) carcinogenesis. Nevertheless, the activity and molecular process of circPRELID2 (hsa_circ_0006528) in RCC progression remain unknown.Methods CircPRELID2, miR-22-3p and ETS variant 1 (ETV1) levels were gauged by qRT-PCR. Effect of the circPRELID2/miR-22-3p/ETV1 axis was evaluated by detecting cell growth, motility, and invasion. Immunoblotting assessed related protein levels. The relationships of circPRELID2/miR-22-3p and miR-22-3p/ETV1 were confirmed by RNA immunoprecipitation (RIP), luciferase reporter or RNA pull-down assay.Results CircPRELID2 was up-regulated in RCC. CircPRELID2 silencing suppressed RCC cell growth, motility and invasion. Moreover, circPRELID2 silencing weakened M2-type macrophage polarization in THP1-induced macrophage cells. CircPRELID2 sequestered miR-22-3p, and circPRELID2 increased ETV1 expression through miR-22-3p. Moreover, the inhibitory impact of circPRELID2 silencing on RCC cell malignant behaviors was mediated by the miR-22-3p/ETV1 axis. Furthermore, circPRELID2 knockdown in vivo hampered growth of xenograft tumors.Conclusion Our study demonstrates that circPRELID2 silencing can mitigate RCC malignant development through the circPRELID2/miR-22-3p/ETV1 axis, highlighting new therapeutic targets for RCC treatment. (1) CircPRELID2 silencing impacts RCC cell phenotypes.(2) CircPRELID2 sequesters miR-22-3p.(3) CircPRELID2 increases ETV1 expression through miR-22-3p.

基金机构：Research project of the Third Affiliated Hospital of Chongqing Medical University

基金资助正文：None.