Giant vesicles form in physiological saline and encapsulate pDNA by the modified electroformation method

作者全名：Zhang, Dingshan; Zhang, Yangruizi; Xiao, Yao; Wang, Yiting; Shen, Yong; Wang, Zhibiao; Qiao, Hai

作者地址：[Zhang, Dingshan; Zhang, Yangruizi; Xiao, Yao; Wang, Yiting; Shen, Yong; Wang, Zhibiao; Qiao, Hai] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Zhang, Dingshan; Zhang, Yangruizi; Xiao, Yao; Wang, Yiting; Wang, Zhibiao; Qiao, Hai] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China; [Shen, Yong] Natl Engn Res Ctr Ultrasound Med, Chongqing 400016, Peoples R China

通信作者：Qiao, H (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China.

来源：COLLOIDS AND SURFACES B-BIOINTERFACES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001219267600001

JCR分区：Q1

影响因子：5.4

年份：2024

卷号：237

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Giant Vesicles; Modified Electroformation; Electrical Impedance; Physiological Saline; Encapsulation

摘要：Giant vesicles (GVs) are used to study the structures and functions of cells and cell membranes. Electroformation is the most commonly used method for GV preparation. However, the electroformation of GVs is hindered in highly concentrated ionic solutions, limiting their application as cell models for research under physiological conditions. In this study, giant multilayer vesicles were successfully generated in physiological saline using a modified electroformation device by adding an insulating layer between the two electrode plates. The influence of the electric frequency and strength on the electroformation of GVs in physiological saline was explored, and a possible mechanism for this improvement was assessed. It has been shown that an insulating layer between the two electrodes can improve the electroformation of GVs in physiological saline by increasing the electrical impedance, which is weakened by the saline solution, thereby restoring the reduced effective electric field strength. Furthermore, macromolecular plasmid DNA (pDNA) was successfully encapsulated in the electroformed GVs of the modified device. This modified electroformation method may be useful for generating eukaryotic cell models under physiological conditions.

基金机构：Natural Science Foundation of Chongqing [cstc2021jcyj-msxmX0550]; Chongqing Talent Plan Project [cstc2021ycjh-bgzxm0021]; CQMU Program for Youth Innovation in Future Medicine [W0155]

基金资助正文：<B>Acknowledgments</B> This work was supported by a grant from the Natural Science Foundation of Chongqing (cstc2021jcyj-msxmX0550) , Chongqing Talent Plan Project (cstc2021ycjh-bgzxm0021) and CQMU Program for Youth Innovation in Future Medicine (W0155) .