A Rapid Self-Assembling Peptide Hydrogel for Delivery of TFF3 to Promote Gastric Mucosal Injury Repair
作者全名：Chen, Jialei; Luo, Jing; Su, Di; Lu, Na; Zhao, Jiawei; Luo, Zhongli
作者地址：[Chen, Jialei; Su, Di; Lu, Na; Zhao, Jiawei; Luo, Zhongli] Chongqing Med Univ, Coll Basic Med Sci, Mol Med & Canc Res Ctr, Chongqing 400016, Peoples R China; [Luo, Jing] Chongqing Med Univ, Dept Pathol & Pathophysiol, Chongqing 400016, Peoples R China
通信作者：Luo, ZL (通讯作者)，Chongqing Med Univ, Coll Basic Med Sci, Mol Med & Canc Res Ctr, Chongqing 400016, Peoples R China.
来源：MOLECULES
ESI学科分类：CHEMISTRY
WOS号：WOS:001220085800001
JCR分区：Q2
影响因子：4.2
年份：2024
卷号：29
期号：9
开始页：
结束页：
文献类型：Article
关键词：self-assembly peptide; TFF3; gastric mucosal injury; gastric mucosal repair; drug delivery
摘要：Self-assembled peptide-based nanobiomaterials exhibit promising prospects for drug delivery applications owing to their commendable biocompatibility and biodegradability, facile tissue uptake and utilization, and minimal or negligible unexpected toxicity. TFF3 is an active peptide autonomously secreted by gastric mucosal cells, possessing multiple biological functions. It acts on the surface of the gastric mucosa, facilitating the repair process of gastric mucosal damage. However, when used as a drug, TFF3 faces significant challenges, including short retention time in the gastric mucosal cavity and deactivation due to degradation by stomach acid. In response to this challenge, we developed a self-assembled short peptide hydrogel, Rqdl10, designed as a delivery vehicle for TFF3. Our investigation encompasses an assessment of its properties, biocompatibility, controlled release of TFF3, and the mechanism underlying the promotion of gastric mucosal injury repair. Congo red/aniline blue staining revealed that Rqdl10 promptly self-assembled in PBS, forming hydrogels. Circular dichroism spectra indicated the presence of a stable beta-sheet secondary structure in the Rqdl10 hydrogel. Cryo-scanning electron microscopy and atomic force microscopy observations demonstrated that the Rqdl10 formed vesicle-like structures in the PBS, which were interconnected to construct a three-dimensional nanostructure. Moreover, the Rqdl10 hydrogel exhibited outstanding biocompatibility and could sustainably and slowly release TFF3. The utilization of the Rqdl10 hydrogel as a carrier for TFF3 substantially augmented its proliferative and migratory capabilities, while concurrently bolstering its anti-inflammatory and anti-apoptotic attributes following gastric mucosal injury. Our findings underscore the immense potential of the self-assembled peptide hydrogel Rqdl10 for biomedical applications, promising significant contributions to healthcare science.
基金机构：Chengdu Sciobio Surgery Institute, China [CSSI-JZ20211021-0002]; National Natural Science Foundation of China [NSFC 31771101]
基金资助正文：This program is supported by the Chengdu Sciobio Surgery Institute, China (Project Number: CSSI-JZ20211021-0002). Zhongli Luo was supported by a grant from the National Natural Science Foundation of China (Project Number: NSFC 31771101).
