Carbon dots-based nanoclusters for sonodynamic therapy of bacterial infection enhanced by deep biofilm penetration and hypoxia alleviation

作者全名：Zhang, Tao; Xing, Haiyan; Xiong, Miao; Gu, Mengqin; Xu, Zhigang; Zhang, Lei; Kang, Yuejun; Xue, Peng

作者地址：[Zhang, Tao; Xing, Haiyan; Xiong, Miao; Xu, Zhigang; Kang, Yuejun; Xue, Peng] Southwest Univ, Sch Mat & Energy, Chongqing 400715, Peoples R China; [Gu, Mengqin] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing 401147, Peoples R China; [Zhang, Lei] Southwest Univ, State Key Lab Resource Insects, Chongqing 400715, Peoples R China

通信作者：Xue, P (通讯作者)，Southwest Univ, Sch Mat & Energy, Chongqing 400715, Peoples R China.

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:001221148800001

JCR分区：Q1

影响因子：13.3

年份：2024

卷号：488

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Sonodynamic therapy; Hypoxia relief; Reactive oxygen species; Deep permeation of drug; Antibacterial infection

摘要：Sonodynamic therapy (SDT) is emerging as a novel and effective treatment modality for antimicrobial applications. However, limited penetration of sonosensitizers and hypoxic infectious microenvironment have significantly restricted the therapeutic efficacy of drug -resistant bacteria. Herein, carbon dots (CDs) -based nanoclusters (MCHH) are facilely constructed by covalent assembly of Mn-doped CDs and simultaneous encapsulation of sonosensitizer hematoporphyrin monomethyl ether (HMME), followed by surface modification with hyaluronic acid (HA). MCHH not only prevents the agglomeration of hydrophobic HMME molecules, but also facilitates their deeper penetration into biofilm. Furthermore, MCHH can be degraded by the overexpressed hyaluronidase in Gram-positive biofilm, resulting in the sustained release of HMME for SDT. Meanwhile, MCHH with catalase-mimic activity contributes to hypoxia alleviation by catalyzing the decomposition of H 2 O 2 into O 2 . Under ultrasound irradiation, intense SDT can be achieved by MCHH, which is enhanced by its high biofilm permeability and local oxygenation. The proposed strategy produces superb bactericidal effect and realizes admirable biofilm disintegration outcome in vitro . On this basis, MCHH administration on bacterial wound infection model expedites wound healing, benefiting from the synergistic action of biofilm eradication, inflammation suppression, increased collagen decomposition and angiogenesis induction. Taken together, this paradigm provides strategic opinions in augmenting SDT efficacy for anti -infective applications.

基金机构：Innovation and Application Development Special Key Project, China [CSTB2023TIAD-LDX0015]; National Key Research and Development Program of China [2023YFF0713900]; Shuangcheng Cooperative Agreement Research Grant of Yibin, China [XNDX2022020013]

基金资助正文：This project was financially supported by National Natural Science Foundation of China (51703186, 32071375) , Chongqing Technologyr Innovation and Application Development Special Key Project, China (CSTB2023TIAD-LDX0015) , National Key Research and Development Program of China (2023YFF0713900) and Shuangcheng Cooperative Agreement Research Grant of Yibin, China (XNDX2022020013) .