Transcriptomics-based identification of TYROBP and TLR8 as novel macrophage-related biomarkers for the diagnosis of acute rejection after kidney transplantation
作者全名：Pei, Jun; Zhang, Jie; Yu, Chengjun; Luo, Jin; Wen, Sheng; Hua, Yi; Wei, Guanghui
作者地址：[Pei, Jun; Zhang, Jie; Yu, Chengjun; Luo, Jin; Wen, Sheng; Hua, Yi; Wei, Guanghui] Chongqing Med Univ, Childrens Hosp, Dept Urol, Chongqing, Peoples R China; [Pei, Jun; Zhang, Jie; Yu, Chengjun; Luo, Jin; Wen, Sheng; Hua, Yi; Wei, Guanghui] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders,Childrens, Minist Educ,Key Lab Child Dev & Disorders, Chongqing Key Lab Pediat,China Int Sci & Technol C, Chongqing, Peoples R China; [Pei, Jun; Zhang, Jie; Yu, Chengjun; Luo, Jin; Wen, Sheng; Hua, Yi; Wei, Guanghui] Children Urogenital Dev & Tissue Engn, Chongqing Key Lab, Chongqing, Peoples R China
通信作者：Hua, Y; Wei, GH (通讯作者)，Chongqing Med Univ, Childrens Hosp, Dept Urol, Chongqing, Peoples R China.
来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ESI学科分类：BIOLOGY & BIOCHEMISTRY
WOS号：WOS:001221762200001
JCR分区：Q3
影响因子：2.5
年份：2024
卷号：709
期号：
开始页：
结束页：
文献类型：Article
关键词：Kidney transplantation; Acute rejection; Macrophages; Immune infiltration
摘要：Macrophages play an important role in the development and progression of acute rejection after kidney transplantation. The study aims to investigate the biological role and significance of macrophage-associated genes (MAG) in acute rejection after kidney transplantation. We utilized transcriptome sequencing results from public databases related to acute rejection of kidney transplantation for comprehensive analysis and validation in animal experiments. We found that a large number of immune-related signaling pathways are activated in acute rejection. PPI protein interaction networks and machine learning were used to establish a Hub gene consisting of TYROBP and TLR8 for the diagnosis of acute rejection. The single-gene GSEA enrichment analysis and immune cell correlation analysis revealed a close correlation between the expression of Hub genes and immune-related biological pathways as well as the expression of multiple immune cells. In addition, the study of TF, miRNAs, and drugs provided a theoretical basis for regulating and treating the Hub genes in acute rejection. Finally, the animal experiments demonstrated once again that acute rejection can aggravate kidney tissue damage, apoptosis level, and increase the release of inflammatory factors. We established and validated a macrophage-associated diagnostic model for acute rejection after kidney transplantation, which can accurately diagnose the biological alterations in acute rejection after kidney transplantation.
基金机构：Funding for children's kidney transplants [YNXM0004]
基金资助正文：This work was supported by Funding for children's kidney transplants (YNXM0004).
