Building a stable and robust anti-interference DNA dissipation system by eliminating the accumulation of systemic specified errors

作者全名：Wang, Luojia; Luo, Wang; Weng, Zhi; Wang, Zhongzhong; Wu, You; Zhao, Rong; Han, Xiaole; Liu, Xin; Zhang, Jianhong; Yang, Yujun; Xie, Guoming

作者地址：[Wang, Luojia; Luo, Wang; Weng, Zhi; Wang, Zhongzhong; Wu, You; Zhao, Rong; Han, Xiaole; Liu, Xin; Zhang, Jianhong; Yang, Yujun; Xie, Guoming] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chongqing Med Lab Microfluid,Chinese Minist Educ, Chongqing 400016, Peoples R China; Chongqing Med Univ, SPRi Engn Res Ctr, Chongqing 400016, Peoples R China

通信作者：Xie, GM (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chongqing Med Lab Microfluid,Chinese Minist Educ, Chongqing 400016, Peoples R China.

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001221850600001

JCR分区：Q1

影响因子：5.7

年份：2024

卷号：1302

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Strand displacement; DNA dissipation; DNA nanotechnology; Nuclease

摘要：Background: The emergence of DNA nanotechnology has enabled the systematic design of diverse bionic dissipative behaviors under the precise control of nucleic acid nanodevices. Nevertheless, when compared to the dissipation observed in robust living systems, it is highly desirable to enhance the anti -interference for artificial DNA dissipation to withstand perturbations and facilitate repairs within the complex biological environments. Results: In this study, we introduce strategically designed " trash cans " to facilitate kinetic control over interferences, transforming the stochastic binding of individual components within a homogeneous solution into a competitive binding process. This approach effectively eliminates incorrect binding and the accumulation of systemic interferences while ensuring a consistent pattern of energy fluctuation from response to silence. Remarkably, even in the presence of numerous interferences differing by only one base, we successfully achieve complete system reset through multiple cycles, effectively restoring the energy level to a minimum. Significance: The system was able to operate stably without any adverse effect under conditions of irregular interference, high -abundance interference, and even multiplex interferences including DNA and RNA crosstalk. This work not only provides an effective paradigm for constructing robust DNA dissipation systems but also greatly broadens the potential of DNA dissipation for applications in high -precision molecular recognition and complex biological reaction networks.

基金机构：National Key R & D Program of China [2022YFC2603800]; National Natural Science Foundation of China [82172369, 82002256]; Top Talent Training Program Project of Chongqing Medical University [BJRC202227]

基金资助正文：This research work was financially supported by the National Key R & D Program of China (2022YFC2603800) , National Natural Science Foundation of China (82172369, 82002256) and Top Talent Training Program Project of Chongqing Medical University (BJRC202227) .