High level 27-HC impairs trophoblast cell invasion and migration via LXR in pre-eclampsia

作者全名：Tong, Hai; Wang, Tingting; Chen, Ruixin; Jin, Pingsong; Jiang, Youqing; Zhang, Xue; Qi, Hongbo

作者地址：[Tong, Hai; Wang, Tingting; Zhang, Xue] Chongqing Med Univ, Dept Obstet, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Tong, Hai; Wang, Tingting; Qi, Hongbo] Chongqing Med Univ, Chongqing Key Lab Maternal & Fetal Med, Chongqing 400016, Peoples R China; [Chen, Ruixin] Sichuan Univ, West China Hosp 2, Dept Gynecol & Obstet, Chengdu 610041, Peoples R China; [Jiang, Youqing] Chongqing Med Univ, Affiliated Hosp 1, Reprod Med Ctr, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Jin, Pingsong; Qi, Hongbo] Chongqing Med Univ CQMU WCH, Women & Childrens Hosp, Dept Obstet & Gynecol, 120 Longshan Rd, Chongqing 401147, Peoples R China; [Qi, Hongbo] Chongqing Med Univ, Chinese Minist Educ, Joint Int Res Lab Reprod & Dev, Chongqing 400016, Peoples R China; [Zhang, Xue] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, 1 Youyi Rd, Chongqing 400016, Peoples R China

通信作者：Qi, HB (通讯作者)，Chongqing Med Univ CQMU WCH, Women & Childrens Hosp, Dept Obstet & Gynecol, 120 Longshan Rd, Chongqing 401147, Peoples R China.; Zhang, X (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, 1 Youyi Rd, Chongqing 400016, Peoples R China.

来源：EXPERIMENTAL CELL RESEARCH

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001222250100001

JCR分区：Q2

影响因子：3.3

年份：2024

卷号：437

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：27-Hydroxycholesterol; LXR; Invasion; Trophoblast; Pre-eclampsia

摘要：Introduction: To explore the potential impact of 27-hydroxycholesterol (27-HC) on trophoblast cell function in pre-eclampsia. Results: The levels of 27-HC and the expression of CYP27A1 are upregulated in clinical samples of PE. Furthermore, high concentrations of 27-HC can inhibit the invasion and migration ability of trophoblast cells in vitro, and this inhibitory effect is weakened after LXR silencing. In HTR8/SVneo cells treated with 27-HC, the expression of ABCA1/ABCG1 are increased. Finally, we established a mouse model of PE using L-NAME (N-NitroL-Arginine Methyl Ester). We found an increase in the levels of 27-HC in the peripheral blood serum of the PE mouse model, and an upregulation of CYP27A1 and LXR expressions in the placenta of the PE mouse model. Conclusion: 27-HC inhibits the invasion and migration ability of trophoblast cells by activating the LXR signaling pathway, which is involved in the pathogenesis of Pre-eclampsia(PE).

基金机构：National Naturel Science Foundation of China [82101777]; Joint Funds of the National Natural Science Foundation of China [U21A20346]; Ministry of Education P.R.C; State Administration of Foreign Experts Affairs P.R.C

基金资助正文：This work was supported by the National Naturel ScienceFoundation of China (No. 82101777) and Joint Funds of the National Natural Science Foundation of China (No. U21A20346) , "111 program (Yuwaiz- huan (2016) 32) " of Ministry of Education P.R.C and State Administration of Foreign Experts Affairs P.R.C.