Mulberry leaf and konjac flour compound dietary fiber improves digestion and metabolism in elderly mice with high-fish-protein diet by regulating gut microbiota structure and intestinal tissue repair

作者全名：Deng, Liling; Zhong, Geng; Wang, Qiong; Zhu, Zhaojing; Peng, Yongbo

作者地址：[Deng, Liling; Wang, Qiong; Zhu, Zhaojing] Chongqing Med & Pharmaceut Coll, Chongqing Engn Res Ctr Pharmaceut Sci, Chongqing Key Lab High Act Tradit Chinese Drug De, Chongqing 401331, Peoples R China; [Deng, Liling; Peng, Yongbo] Chongqing Med Univ, Coll Pharm, Key Lab Biochem & Mol Pharmacol, Chongqing Key Lab Pharmaceut Metab Res, Chongqing 400016, Peoples R China; [Zhong, Geng] Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China

通信作者：Deng, LL; Peng, YB (通讯作者)，Chongqing Med & Pharmaceut Coll, Chongqing Engn Res Ctr Pharmaceut Sci, Chongqing Key Lab High Act Tradit Chinese Drug De, Chongqing 401331, Peoples R China.

来源：FOOD CHEMISTRY-X

ESI学科分类：　

WOS号：WOS:001222761300001

JCR分区：Q1

影响因子：6.5

年份：2024

卷号：22

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Mulberry leaf and konjac flour compound; dietary fiber; Elderly mice; Protein metabolism; Gut microbiota; Gastrointestinal tissue

摘要：Ensuring sufficient protein intake, efficient digestion, and optimal absorption are crucial for the elderly. This study aims to investigate the potential of a compound dietary fiber, consisting of mulberry leaf and konjac flour (CMK), to enhance the digestion and absorption of a high -fish -protein diet in elderly mice. Results showed that CMK effectively reduced the number of unique peptide segments, generated short -chain fatty acids (SCFA) in feces, improved the content of glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), amino acid, and urea nitrogen in serum, activated the contents of pepsin, trypsin, and erepsin, and enhanced the expression of glutamate dehydrogenase (GDH), peptide transporter 1 (PepT1), and aminopeptidase N (APN). Furthermore, CMK demonstrated its ability to decrease the levels of tumor necrosis factor- alpha (TNF- alpha), interleukin1 beta (IL-1 beta ), interleukin-10 (IL -10), lipopolysaccharide (LPS), and lipopolysaccharide binding protein (LBP), while increase the abundance of beneficial bacteria, such as Lactobacillus and Blautia . In conclusion, CMK proved effective in enhancing the digestion and metabolism of protein in elderly mice through the regulation of gut microbiota structure and intestinal tissue repair.

基金机构：China Postdoctoral Science Foun-dation [2023MD734133]; Special Funding for Postdoctoral Research Projects in Chongqing [2022CQBSHTB2009]; School level Project of Chongqing Medical and Pharmaceutical College [ygz2022103]

基金资助正文：<B>Acknowledgements</B> This work was supported by the China Postdoctoral Science Foun-dation (2023MD734133) , the Special Funding for Postdoctoral Research Projects in Chongqing (2022CQBSHTB2009) , and the School level Project of Chongqing Medical and Pharmaceutical College (ygz2022103) .