Unraveling the mechanism in l-Caldesmon regulating the osteogenic differentiation of PDLSCs: An innovative perspective

作者全名：Li, Yuejia; Mei, Ziyi; Deng, Pingmeng; Zhou, Sha; Qian, Aizhuo; Zhang, Xiya; Li, Jie

作者地址：[Li, Yuejia; Mei, Ziyi; Deng, Pingmeng; Zhou, Sha; Qian, Aizhuo; Zhang, Xiya; Li, Jie] Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China; [Li, Yuejia; Mei, Ziyi; Deng, Pingmeng; Zhou, Sha; Qian, Aizhuo; Zhang, Xiya; Li, Jie] Chongqing Med Univ, Chongqing Key Lab Oral Dis, Chongqing, Peoples R China; [Li, Yuejia; Mei, Ziyi; Deng, Pingmeng; Zhou, Sha; Qian, Aizhuo; Zhang, Xiya; Li, Jie] Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China

通信作者：Li, J (通讯作者)，Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China.

来源：CELLULAR SIGNALLING

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001223365900001

JCR分区：Q2

影响因子：4.4

年份：2024

卷号：118

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：BMP2; Bone regeneration; CALD1; Osteogenic differentiation; PDLSCs; Wnt signaling pathway

摘要：Maxillofacial bone defect is one of the common symptoms in maxillofacial, which affects the function and aesthetics of maxillofacial region. Periodontal ligament stem cells (PDLSCs) are extensively used in bone tissue engineering. The mechanism that regulates the osteogenic differentiation of PDLSCs remains not fully elucidated. Previous studies demonstrated that l-Caldesmon (l-CALD, or CALD1) might be involved in the osteogenic differentiation of PDLSCs. Here, the mechanism by which CALD1 regulates the osteogenic differentiation of PDLSCs is investigated. The osteogenic differentiation of PDLSCs is enhanced with Cald1 knockdown. Whole transcriptome sequencing (RNA-seq) analysis shows that bone morphogenetic proteins (BMP) signaling pathway and Wingless type (Wnt) pathway have significant change with Cald1 knockdown, and the expressions of Wntinduced secreted protein 1 (WISP1), BMP2, Smad1/5/9, and p-Smad1/5/9 are significantly upregulated, while Glycogen synthase kinase 3 beta (GSK3 beta) and p-GSK3 beta are downregulated. In addition, subcutaneous implantation in nude mice shows that knockdown of Cald1 enhances the osteogenic differentiation of PDLSCs in vivo. Taken together, this study demonstrates that knockdown of Cald1 enhances the osteogenic differentiation of PDLSCs by BMP and Wnt signaling pathways, and provides a novel approach for subsequent clinical treatment.

基金机构：National Natural Science Foundation of China [82071072, 81700932]; Natural Science Founda-tion of Chongqing [CSTB2022NSCQ-LZX0004]; CQMU Program for Youth Innovation in Future Medicine [W0179]

基金资助正文：<B>Funding</B> This work was supported by the National Natural Science Foundation of China [Grant No. 82071072 & 81700932] , Natural Science Founda-tion of Chongqing [Grant No. CSTB2022NSCQ-LZX0004] , and CQMU Program for Youth Innovation in Future Medicine (Grant No. W0179) .