Neuroprotective effects of cordycepin inhibit glutamate-induced apoptosis in hippocampal neurons

作者全名：Sun, Huizhen; Wei, Shanshan; Gong, Yanchun; Ding, Kaizhi; Tang, Shan; Sun, Wei; Yuan, Chunhua; Huang, Liping; Liu, Zhibing; Chen, Chong; Yao, Lihua

作者地址：[Sun, Huizhen; Wei, Shanshan; Gong, Yanchun; Ding, Kaizhi; Tang, Shan; Sun, Wei; Yuan, Chunhua; Chen, Chong; Yao, Lihua] Jiangxi Sci & Technol Normal Univ, Sch Life Sci, Nanchang, Jiangxi, Peoples R China; [Sun, Huizhen] Shan Cty Renmin Rd Primary Sch, Heze, Shandong, Peoples R China; [Gong, Yanchun; Tang, Shan; Liu, Zhibing; Chen, Chong; Yao, Lihua] Jiangxi Sci & Technol Normal Univ, Sch Phys Educ & Hlth, Nanchang, Jiangxi, Peoples R China; [Huang, Liping] Jiangxi Univ Tradit Chinese Med, Sch Pharm, Nanchang, Jiangxi, Peoples R China; [Chen, Chong] Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Affiliated Hosp 1, Chongqing, Peoples R China

通信作者：Chen, C; Yao, LH (通讯作者)，Jiangxi Sci & Technol Normal Univ, Sch Life Sci, Nanchang, Jiangxi, Peoples R China.; Chen, C; Yao, LH (通讯作者)，Jiangxi Sci & Technol Normal Univ, Sch Phys Educ & Hlth, Nanchang, Jiangxi, Peoples R China.; Chen, C (通讯作者)，Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Affiliated Hosp 1, Chongqing, Peoples R China.

来源：CELL STRESS & CHAPERONES

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001223781000001

JCR分区：Q3

影响因子：3.3

年份：2024

卷号：29

期号：1

开始页：10

结束页：20

文献类型：Article

关键词：Cordycepin; Neuroprotection; Apoptosis; Glutamate; Adenosine A1 receptor

摘要：Glutamate is a neurotransmitter that can cause excitatory neurotoxicity when its extracellular concentration is too high, leading to disrupted calcium balance and increased production of reactive oxygen species (ROS). Cordycepin, a nucleoside adenosine derivative, has been shown to protect against excitatory neurotoxicity induced by glutamate. To investigate its potential neuroprotective effects, the present study employed fluorescence detection and spectrophotometry techniques to analyze primary hippocampal-cultured neurons. The results showed that glutamate toxicity reduced hippocampal neuron viability, increased ROS production, and increased intracellular calcium levels. Additionally, glutamate-induced cytotoxicity activated acetylcholinesterase and decreased glutathione levels. However, cordycepin inhibited glutamate-induced cell death, improved cell viability, reduced ROS production, and lowered Ca2+ levels. It also inhibited acetylcholinesterase activation and increased glutathione levels. This study suggests that cordycepin can protect against glutamate-induced neuronal injury in cell models, and this effect was inhibited by adenosine A1 receptor blockers, indicating that its neuroprotective effect is achieved through activation of the adenosine A1 receptor.

基金机构：National Natural Science Foundation of China [31960193, 31660275]; Jiangxi "Double Thousand Plan" Cultivation Program for Distinguished Talents in Scientific and Technological Innovation [jxsq2019201011]; Natural Science Foundation of Jiangxi Province [20202ACBL206029]; Key Construction Laboratory Program of Jiangxi Science and Technology Normal University [2017ZDPYJD004]; Academic Leader Project of Jiangxi Province [20182BCB22005]

基金资助正文：This work was supported by the National Natural Science Foundation of China (31960193, 31660275) , Jiangxi "Double Thousand Plan" Cultivation Program for Distinguished Talents in Scientific and Technological Innovation to Lihua Yao (jxsq2019201011) , Natural Science Foundation of Jiangxi Province (20202ACBL206029) , Key Construction Laboratory Program of Jiangxi Science and Technology Normal University (2017ZDPYJD004) , The Academic Leader Project of Jiangxi Province (20182BCB22005) .