Adolescent male rats show altered gut microbiota composition associated with depressive-like behavior after chronic unpredictable mild stress: Differences from adult rats

作者全名：Wang, Jie; Fan, Li; Teng, Teng; Wu, Hongyan; Liu, Xueer; Yin, Bangmin; Li, Xuemei; Jiang, Yuanliang; Zhao, Jianting; Wu, Qingyuan; Guo, Yi; Zhou, Xinyu; Xie, Peng

作者地址：[Wang, Jie; Teng, Teng; Wu, Hongyan; Liu, Xueer; Yin, Bangmin; Li, Xuemei; Jiang, Yuanliang; Zhou, Xinyu] Chongqing Med Univ, Dept Gastroenterol, Affiliated Hosp 1, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Wang, Jie; Fan, Li; Teng, Teng; Wu, Hongyan; Liu, Xueer; Yin, Bangmin; Li, Xuemei; Jiang, Yuanliang; Zhou, Xinyu; Xie, Peng] Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Affiliated Hosp 1, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Fan, Li; Xie, Peng] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing, Peoples R China; [Zhao, Jianting] Xinxiang Med Coll, Clin Coll 4, Xinxiang Med Coll, Dept Neurol, Xinxiang, Peoples R China; [Wu, Qingyuan] Chongqing Univ, Three Gorges Hosp, Dept Neurol, Chongqing, Peoples R China; [Guo, Yi] Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Dept Neurol,Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China

通信作者：Zhou, XY (通讯作者)，Chongqing Med Univ, Dept Gastroenterol, Affiliated Hosp 1, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China.; Zhou, XY; Xie, P (通讯作者)，Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Affiliated Hosp 1, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China.; Xie, P (通讯作者)，Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing, Peoples R China.

来源：JOURNAL OF PSYCHIATRIC RESEARCH

ESI学科分类：PSYCHIATRY/PSYCHOLOGY

WOS号：WOS:001224016100001

JCR分区：Q1

影响因子：3.7

年份：2024

卷号：173

期号：　

开始页：183

结束页：191

文献类型：Article

关键词：Depression; Chronic unpredictable mild stress; Adolescent; Adult; Gut microbiota

摘要：Accumulating evidence reveals the metabolism and neurotransmitter systems are different in major depressive disorder (MDD) between adolescent and adult patients; however, much is still unknown from the gut microbiome perspective. To minimize confounding factors such as geographical location, ethnicity, diet, and drugs, we investigated the gut microbial differences between adolescent and adult male Sprague-Dawley rats. We exposed the adolescent rats to chronic unpredictable mild stress (CUMS) for 3 weeks and assessed their behavior using the sucrose preference test (SPT), open field test (OFT), and forced swimming test (FST). We collected and sequenced fecal samples after the behavioral tests and compared them with our previous data on adult rats. Both adolescent and adult CUMS rats exhibited reduced sucrose preference in SPT, reduced total distance in OFT, and increased immobility time in FST. Moreover, compared to their respective controls, the adolescent CUMS rats had distinct amplicon sequence variants (ASVs) mainly in the Muribaculaceae family, Bacteroidetes phylum, while the adult CUMS rats had those in the Lachnospiraceae family, Firmicutes phylum. In the adolescent group, the Muribaculaceae negatively correlated with FST and positively correlated with SPT and OFT. In the adult group, the different genera in the Lachnospiraceae showed opposite correlations with FST. Furthermore, the adolescent CUMS rats showed disrupted microbial functions, such as "Xenobiotics biodegradation and metabolism" and "Immune system", while the adult CUMS rats did not. These results confirmed the gut microbiota differences between adolescent and adult rats after CUMS modeling and provided new insight into the age-related influence on depression models.

基金机构：STI2030-Major Projects [2022ZD0212900]; National Natural Science Foundation Project of China [81820108015]; Chongqing medical scientific research project (Joint project of Chongqing Health Commission and Science and Technology Bureau) [2023CCXM003]; National Basic Research Program of China [2017YFA0505700]; National Natural Science Foundation Project of China (General Program) [82271565]; Chongqing Science and Technology Commission [cstc2020jcyj-jqX0024]

基金资助正文：This study was supported by the STI2030-Major Projects (Grant no.2022ZD0212900 to XZ) , the National Natural Science Foundation Project of China (Grant no. 81820108015 to PX) , Chongqing medical scientific research project (Joint project of Chongqing Health Commission and Science and Technology Bureau) (Grant no. 2023CCXM003 to PX) , the National Basic Research Program of China (Grant no. 2017YFA0505700 to PX) , the National Natural Science Foundation Project of China (General Program) (Grant no. 82271565 to XZ) , the institutional funds from the Chongqing Science and Technology Commission (Grant no. cstc2020jcyj-jqX0024 to XZ) .