Soft interface confined DNA walker for sensitive and specific detection of SARS-CoV-2 variants

作者全名：Wang, Zhangmin; Yuan, Zuowei; Liu, Min; Liu, Zhidong; Leng, Ping; Ding, Shijia; Guo, Jinlin; Zhang, Juan

作者地址：[Liu, Min; Guo, Jinlin; Zhang, Juan] Chengdu Univ Tradit Chinese Med, Coll Med Technol, Chengdu 611137, Peoples R China; [Liu, Zhidong; Guo, Jinlin; Zhang, Juan] Chongqing Hosp Tradit Chinese Med, Dept Lab Med, Chongqing 400021, Peoples R China; [Zhang, Juan] Chongqing Key Lab Sichuan Chongqing Coconstruct Di, Chongqing 400021, Peoples R China; [Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China

通信作者：Zhang, J (通讯作者)，Chengdu Univ Tradit Chinese Med, Coll Med Technol, Chengdu 611137, Peoples R China.

来源：TALANTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001224562700001

JCR分区：Q1

影响因子：5.6

年份：2024

卷号：274

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：DNA walker; Soft interface; Catalytic hairpin assembly; SARS-CoV-2 RNA

摘要：Nucleic acid detection is conducive to preventing the spread of COVID-19 pandemic. In this work, we successfully designed a soft interface confined DNA walker by anchoring hairpin reporter probes on cell membranes for the detection of SARS-CoV-2 variants. In the presence of target RNA, the cyclic self -assembly reaction occurred between hairpin probes H1 and H2, and the continuous walking of target RNA on cell membranes led to the gradual amplification of fluorescence signal. The enrichment of H1 on membranes and the unique fluidity of membranes promoted the collision efficiency between DNA strands in the reaction process, endowing this method with high sensitivity. In addition, the double-blind test of synthetic RNA in 5% normal human serum demonstrated the good stability and anti -interference in complex environment of this method, which exhibited great potential in clinical diagnostics.

基金机构：Chongqing Natural Science Foundation Joint Fund for Innovation and Development [81960391]; [CSTB2023NSCQ-LZX0007]

基金资助正文：This work was financially supported by Chongqing scientific research institutions performance incentive and guidance project (cstc2021jxjl130038) , National Natural Science Foundation of China (81960391) , and Chongqing Natural Science Foundation Joint Fund for Innovation and Development (CSTB2023NSCQ-LZX0007) .r (81960391) , and Chongqing Natural Science Foundation Joint Fund for Innovation and Development (CSTB2023NSCQ-LZX0007) .