Identifying endoplasmic reticulum stress-related genes as new diagnostic and prognostic biomarkers in clear cell renal cell carcinoma

作者全名："Yu, Haitao; Zhang, Chunlin; Bai, Xuesong; Yin, Hubin; Li, Xinyuan; Zhou, Xiang; He, Weiyang; Kuang, Youlin; Gou, Xin; Li, Jie"

作者地址："[Yu, Haitao; Zhang, Chunlin; Bai, Xuesong; Yin, Hubin; Li, Xinyuan; Zhou, Xiang; He, Weiyang; Kuang, Youlin; Gou, Xin; Li, Jie] Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, 1 Youyi Rd, Chongqing 400010, Peoples R China; [Yu, Haitao; Zhang, Chunlin; Yin, Hubin; Li, Xinyuan; Zhou, Xiang] Chongqing Med Univ, Affiliated Hosp 1, Chongqing Key Lab Mol Oncol & Epigenet, Chongqing, Peoples R China"

通信作者："Gou, X; Li, J (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, 1 Youyi Rd, Chongqing 400010, Peoples R China."

来源：TRANSLATIONAL ANDROLOGY AND UROLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001225093600001

JCR分区：Q3

影响因子：2

年份：2024

卷号：13

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Clear cell renal cell carcinoma (ccRCC); endoplasmic reticulum stress (ERS); diagnosis; prognosis

摘要："Background: Clear cell renal cell carcinoma (ccRCC) is one of the most common cancers worldwide, and its incidence is increasing every year. Endoplasmic reticulum stress (ERS) caused by protein misfolding has broad and profound effects on the progression and metastasis of various cancers. Accumulating evidence suggests that ERS is closely related to the occurrence and progression of ccRCC. This study aimed to identify ERS-related genes for evaluating the prognosis of ccRCC. Methods: Transcriptomic expression profiles were obtained from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA), and clinical data were downloaded from the TCGA. First, the differentially expressed genes (DEGs) were analyzed using the limma package, and the DEGs related to ERS (ERS-DEGs) were identified from the GeneCards database. Second, a function and pathway enrichment analysis and a Gene Set Enrichment Analysis (GSEA) were performed. Third, a protein-protein interaction (PPI) network was constructed to identify the hub genes, and a gene-micro RNA (miRNA) network and gene-transcription factor (TF) network were established using the hub genes. Finally, a least absolute shrinkage and selection operator (LASSO) regression analysis was conducted to establish a diagnostic model, and a Cox analysis was used to analyze the correlations between the expression of the characteristic genes and the clinical characteristics. Results: We identified 11 signature genes and established a diagnostic model. Further, the Cox analysis results revealed a correlation between the expression levels of the signature genes and the clinical characteristics. Ultimately, five signature genes (i.e., TNFSF13B, APOL1, COL5A3, and CDH5) were found to be associated with a poor prognosis. Conclusions: This study suggests that TNFSF13B, APOL1, COL5A3, and CDH5 may have potential as prognostic biomarkers in ccRCC and may provide new evidence to support targeted therapy in ccRCC."

基金机构：Chongqing Young and Middle-Aged High -End Medical Talents Project Fund

基金资助正文：Funding: This work was supported by the Chongqing Young and Middle-Aged High -End Medical Talents Project Fund.