Green Synthesis of Gold Nanoparticles from Polygahatous Polysaccharides and Their Anticancer Effect on Hepatic Carcinoma through Immunoregulation

作者全名：Leng, Maodong; Jiang, Huiqin; Zhang, Sitong; Bao, Yixi

作者地址：[Leng, Maodong; Zhang, Sitong; Bao, Yixi] Chongqing Med Univ, Affiliated Hosp 2, Dept Clin Lab, Chongqing 400010, Peoples R China; [Leng, Maodong] Zhengzhou Univ, Childrens Hosp, Dept Clin Lab, Zhengzhou Key Lab Childrens Infect & Immun, Zhengzhou 450018, Henan, Peoples R China; [Jiang, Huiqin] Zhengzhou Univ, Inst Adv Biomed Sci, Innovat Ctr Basic Res Metab Associated Fatty Liver, Minist Educ China,Tianjian Lab Adv Biomed Sci, Zhengzhou 450000, Henan, Peoples R China

通信作者：Bao, YX (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Clin Lab, Chongqing 400010, Peoples R China.

来源：ACS OMEGA

ESI学科分类：CHEMISTRY

WOS号：WOS:001225457500001

JCR分区：Q2

影响因子：3.7

年份：2024

卷号：9

期号：19

开始页：21144

结束页：21151

文献类型：Article

关键词：　

摘要：Hepatic carcinoma is one of the leading causes of morbidity and mortality among all cancers, but no effective treatment measures have been developed. Herein, polystyrene polysaccharide (PSP) extracted from Polygonatum was used to synthesize gold nanoparticles (PSP-AuNPs) by heating and reduction methods, and the characteristics of the PSP-AuNPs were detected after successful synthesis. In vitro, the immunoregulatory effects of PSP-AuNPs were studied by testing the concentrations of NO, TNF-alpha, and IL-12p70 in the culture media of PSP-AuNPs-treated RAW264.7 macrophages, and the effect of biocompatibility on the viability of RAW264.7 macrophages and L02 cells was studied via a CCK-8 assay. In vivo, tumor-bearing mice were established and treated with PSP-AuNPs, and the anticancer effects were studied by detecting trends in tumor volume, tumor inhibition rate, and tumor cell proliferation index. Immunoregulation was assessed by evaluating the serum levels of TNF-alpha and IL-10, the CD4+/CD8+ lymphocyte ratio in peripheral blood and the spleen and thymus indices; toxicity was investigated by measuring body weight, liver and renal function indices. The results showed that PSP-AuNPs could regulate immune function both in vitro and in vivo with almost no toxicity. PSP-AuNPs exhibited excellent anticancer effects on hepatic carcinoma in vivo. The anticancer effect could be strengthened, and the toxicity could be reduced by the combined use of PSP-AuNPs and ADM. In conclusion, PSP-AuNPs could be effective as a therapy and adjuvant therapy for treating hepatic carcinoma, providing potential treatment strategies for this disease.

基金机构：Tianjian Laboratory of Advanced Biomedical Sciences; Pediatric Medical Research Institute of Children's Hospital Affiliated to Zhengzhou University

基金资助正文：The authors thank the Pediatric Medical Research Institute of Children's Hospital Affiliated to Zhengzhou University and Academy of Medical Sciences of Zhengzhou University for providing laboratories and instruments to perform the experiments.