miR-200b-3p relieved inflammation in patients with heart failure by regulating ZEB1 expression

作者全名：Wei, Bo; Li, Zhiyong; Wang, Li; Zhang, Haitao; Gou, Wen

作者地址：[Wei, Bo; Li, Zhiyong; Wang, Li; Zhang, Haitao; Gou, Wen] Chongqing Med Univ, Dept Cardiol, Yongchuan Hosp, 439,Xuanhua Rd, Chongqing 402177, Peoples R China

通信作者：Gou, W (通讯作者)，Chongqing Med Univ, Dept Cardiol, Yongchuan Hosp, 439,Xuanhua Rd, Chongqing 402177, Peoples R China.

来源：JOURNAL OF CARDIOTHORACIC SURGERY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001225958700003

JCR分区：Q3

影响因子：1.5

年份：2024

卷号：19

期号：1

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结束页：　

文献类型：Article

关键词：Heart failure; MiR-200b-3p; ZEB1; Inflammatory response; ROC curve

摘要：Background MicroRNA-200b-3p (miR-200b-3p) plays a pivotal role in inflammatory responses and is implicated in various inflammatory disorders. In this study, we aim to explore the role of miR-200b-3p in the inflammatory response in heart failure (HF).Methods Patients diagnosed with heart failure and age-matched healthy controls were studied. Peripheral blood samples from participants were collected for RNA-seq analysis to explore the expression profile of miR-200b-3p. The predictive value of miR-200b-3p and ZEB1 in the prognosis of heart failure was evaluated by analyzing the receiver operating characteristic (ROC) curve. Bioinformatics analysis and double luciferase reporter gene analysis were used to confirm the interaction between miR-200b-3p and ZEB1. Real-time quantitative polymerase chain reaction (QRT-PCR) was used to detect the expression levels of miR-200b-3p and ZEB1 in cardiopulmonary bypass. Additionally, the effects of miR-200b-3p on myocardial cell line (H9c2) injury were evaluated by enzyme-linked immunosorbent assay (ELISA).Results In the extracardiac circulation of HF patients, miR-200b-3p expression was significantly reduced, while ZEB1 levels were notably elevated. Analysis of the ROC curve revealed that miR-200b-3p and ZEB1 have predictive value in the prognosis of HF patients. The double luciferase reporter experiment demonstrated that miR-200b-3p binds to ZEB1 and inhibits its expression. Overexpression of miR-200b-3p demonstrated a remarkable ability to alleviate inflammation and inhibit the damage to myocardial cells in vivo.Conclusion MiR-200b-3p can target and inhibit ZEB1, reducing the inflammatory reaction of myocardial cells. The miR-200b-3p/ZEB1 network may be helpful in preventing and treating HF.

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