Chemokine receptor 7 contributes to T- and B-cell filtering in ageing bladder, cystitis and bladder cancer

作者全名：Zhao, Jiang; Luo, Xing; Yang, Chengfei; Yang, Xiao; Deng, Min; Sun, Bishao; Zhu, Jingzhen; Dong, Zongming; Wang, Yangcai; Li, Jia; Yang, Xingliang; Li, Benyi; Wang, Xiangwei; Zheng, Ji

作者地址：[Zhao, Jiang; Wang, Xiangwei] Guangdong Med Univ, Affiliated Hosp, Dept Urol, Zhanjiang 524001, Peoples R China; [Zhao, Jiang; Luo, Xing; Deng, Min; Sun, Bishao; Zhu, Jingzhen; Dong, Zongming; Wang, Yangcai; Li, Jia; Yang, Xingliang; Zheng, Ji] Army Mil Med Univ, Affiliated Hosp 2, Dept Urol, Chongqing 400037, Peoples R China; [Zhao, Jiang; Li, Benyi] Univ Kansas, Med Ctr, Dept Urol, Kansas City, KS 66160 USA; [Yang, Chengfei] Army Mil Med Univ, Affiliated Hosp 2, Dept Thorac Surg, Chongqing 400037, Peoples R China; [Yang, Xiao] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China

通信作者：Zhao, J; Wang, XW (通讯作者)，Guangdong Med Univ, Affiliated Hosp, Dept Urol, Zhanjiang 524001, Peoples R China.; Zhao, J; Zheng, J (通讯作者)，Army Mil Med Univ, Affiliated Hosp 2, Dept Urol, Chongqing 400037, Peoples R China.; Zhao, J; Li, BY (通讯作者)，Univ Kansas, Med Ctr, Dept Urol, Kansas City, KS 66160 USA.

来源：IMMUNITY & AGEING

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001227235700001

JCR分区：Q1

影响因子：5.2

年份：2024

卷号：21

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Ageing bladder; Interstitial cystitis/bladder pain syndrome; Bladder urothelial carcinoma; Immune microenvironment; CCR7; Immune cell infiltration; T-cell; B-cell

摘要：Background Research has suggested significant correlations among ageing, immune microenvironment, inflammation and tumours. However, the relationships among ageing, immune microenvironment, cystitis and bladder urothelial carcinoma (BLCA) in the bladder have rarely been reported. Methods Bladder single-cell and transcriptomic data from young and old mice were used for immune landscape analysis. Transcriptome, single-cell and The Cancer Genome Atlas Program datasets of BLCA and interstitial cystitis/bladder pain syndrome (IC/BPS) were used to analyse immune cell infiltration and molecular expression. Bladder tissues from mice, IC/BPS and BLCA were collected to validate the results. Results Eight types of immune cells (macrophages, B-cells, dendritic cells, T-cells, monocytes, natural killer cells, gamma delta T-cells and ILC2) were identified in the bladder of mice. Aged mice bladder tissues had a significantly higher number of T-cells, gamma delta T-cells, ILC2 and B-cells than those in the young group (P < 0.05). Three types of T-cells (NK T-cells, gamma delta T-cells and na & iuml;ve T-cells) and three types of B-cells (follicular B-cells, plasma and memory B-cells) were identified in aged mice bladder. Chemokine receptor 7 (CCR7) is highly expressed in aged bladder, IC/BPS and BLCA (P < 0.05). CCR7 is likely to be involved in T- and B-cell infiltration in aged bladder, IC/BPS and BLCA. Interestingly, the high CCR7 expression on BLCA cell membranes was a prognostic protective factor. Conclusions In this study, we characterised the expression profiles of immune cells in bladder tissues of aged and young mice and demonstrated that CCR7-mediated T- and B-cell filtration contributes to the development of bladder ageing, IC/BPS and BLCA.

基金机构：Chongqing Key Natural Science Foundation [CSTB2023NSCQ-ZDJ0013]; Top Young and Middle-aged Medical Talent of Chongqing; Guangdong Provincial Natural Science Foundation General Project [2414050006122]; Second Affiliated Hospital of the Army Military Medical University "Qingbo Program" Key Projects [2023YQB018]

基金资助正文：This study was supported by the Chongqing Key Natural Science Foundation (CSTB2023NSCQ-ZDJ0013) and the Top Young and Middle-aged Medical Talent of Chongqing and the Guangdong Provincial Natural Science Foundation General Project (2414050006122) and the Second Affiliated Hospital of the Army Military Medical University "Qingbo Program" Key Projects (2023YQB018).