Surface confinement-induced electrochemiluminescence enhancement of Au nanoclusters for ultrasensitive detection of a soluble urokinase-type plasminogen activator receptor
作者全名：Chen, Xiaorui; Shi, Xiaoyu; Tan, Yue; Wang, Jiaxin; Zeng, Saiyi; Yuan, Ruo; Chen, Yunqing
作者地址：[Chen, Xiaorui] Chongqing Med Univ, Dept Pulm & Crit Care Med, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Tan, Yue; Wang, Jiaxin; Chen, Yunqing] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Zeng, Saiyi] Chongqing Med Univ, Clin Coll 2, Chongqing 400010, Peoples R China; [Shi, Xiaoyu; Yuan, Ruo] Southwest Univ, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China
通信作者：Chen, YQ (通讯作者)，Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China.; Yuan, R (通讯作者)，Southwest Univ, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China.
来源：SENSORS AND ACTUATORS B-CHEMICAL
ESI学科分类：CHEMISTRY
WOS号：WOS:001227601600001
JCR分区：Q1
影响因子：8
年份：2024
卷号：411
期号：
开始页：
结束页：
文献类型：Article
关键词：Gold nanocluster; Zn-containing hydroxyl double salt; Confinement-induced electrochemiluminescence; Biosensor; Soluble urokinase type plasminogen activator receptor
摘要：In this study, gold nanoclusters (Au NCs) anchored on the surface of 2D Zn-containing hydroxyl double salt (ZnHDS) with surface confinement - induced electrochemiluminescence (SC-ECL) improvement were used as an intensive ECL emitter to construct a biosensor for ultrasensitive detection of soluble urokinase-type plasminogen activator receptor (suPAR) related to cardiovascular disease in chronic nephritis patients. Impressively, SC-ECL of Au NCs/Zn-HDS nanocomposites exhibited fast electrochemical excitation and efficient radiation transition, showing 4.9 times stronger ECL emission and 10.9 times higher ECL efficiency than those of pure Au NCs. This improvement was attributed to the matched energy level of Zn-HDS and Au NCs, enabling electron - hole pairs of Au NCs to be trapped by the energy levels of Zn-HDS. Moreover, a bipedal DNA walker was generated through target-induced DNA strand displacement and catalytic hairpin assembly (CHA), which was attached to a stable DNA cubic track through electrostatic attraction of positively charged SH-PEG-NH 3 + to improve walking efficiency and further boost detection sensitivity. Consequently, the proposed sensing platform achieved trace detection of suPAR ranging from 0.700 pg/mL to 80.0 ng/mL with a low detection limit of 0.344 pg/mL and was successfully applied to detect suPAR in patient serum. This work synthesized a highly efficient metal nanoclusterbased ECL emitter, showing great potential in constructing ultrasensitive biosensors for early disease diagnosis.
基金机构：National Natural Science Foundation of China [82270490]; Health Appropriate Technology Promotion Project of Chongqing Municipal Health Commission [2020jstg020]
基金资助正文：This work was financially supported by National Natural Science Foundation of China (82270490) and Health Appropriate Technology Promotion Project of Chongqing Municipal Health Commission (2020jstg020) .
