"Comprehensive quality evaluation of <i>Lysimachia christinae</i> Hance via fingerprint, spectrum-effect relationship, and quantitative analyses of multiple components by single marker"

作者全名："Luorong, Quji; Tan, Li-Hong; Yu, Bao; Wu, Yingqin; Luo, Juan; Cao, Wei-Guo; Li, Jingjing; Chen, Huan; Zhang, Dan"

作者地址："[Luorong, Quji; Wu, Yingqin; Luo, Juan; Chen, Huan; Zhang, Dan] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing, Peoples R China; [Tan, Li-Hong] Chongqing Med & Pharmaceut Coll, Coll Pharm & Coll Tradit Chinese Med, Chongqing, Peoples R China; [Yu, Bao; Cao, Wei-Guo; Li, Jingjing; Chen, Huan; Zhang, Dan] Chongqing Coll Tradit Chinese Med, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China"

通信作者："Chen, H; Zhang, D (通讯作者)，Chongqing Coll Tradit Chinese Med, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China."

来源：PHYTOCHEMICAL ANALYSIS

ESI学科分类：PLANT & ANIMAL SCIENCE

WOS号：WOS:001228295800001

JCR分区：Q2

影响因子：3.3

年份：2024

卷号：35

期号：6

开始页：1527

结束页：1536

文献类型：Article

关键词：anti-inflammatory; choleretic; fingerprint techniques; Lysimachia christinae Hance; quality evaluation

摘要："Background: Lysimachia christinae Hance (LCH) is a traditional medicine used to treat gallstone disease and cholecystitis. Despite its known anti-inflammatory and choleretic effects, its quality has not been extensively evaluated. Objective: In this study, we aimed to establish a reliable quality evaluation method for LCH via fingerprint, spectrum-effect relationship, and quantitative analyses of multicomponents by a single marker (QAMS). Methods: First, the fingerprints and anti-inflammatory and choleretic activities of 14 LCH batches were determined. Then, the gray relation analysis method was used to analyze the peak areas of the fingerprint profile and pharmacodynamic data. Subsequently, the characteristic peaks were tentatively identified using high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Finally, rutin was selected as the internal reference material, and QAMS was used to analyze the LCH components. Results: Pharmacodynamic experiments confirmed that LCH exerted anti-inflammatory and choleretic effects. Moreover, 15 flavonoids related to the anti-inflammatory and choleretic effects of LCH were identified. Notably, relative error percentage between the QAMS and external standard method was less than 5%. Conclusion: This study successfully established a comprehensive evaluation method for the qualitative and quantitative analyses of LCH."

基金机构："Chongqing Science and Technology Bureau; Chongqing Graduate Student Research Innovation Project [KJZD-M202215102, KJQN202215118, 2023-23-14]; [cstc2020jscx-dxwtBX0054]; [CSTB2022NSCQ-MSX1646]"

基金资助正文："This research was founded by the Chongqing Science and Technology Bureau (cstc2020jscx-dxwtBX0054, CSTB2022NSCQ-MSX1646), Chongqing Graduate Student Research Innovation Project (KJZD-M202215102, KJQN202215118), and Chongqing QiHuang scholars support project (2023-23-14)."