Transcriptional regulation of TacL-mediated lipoteichoic acids biosynthesis by ComE during competence impacts pneumococcal transformation
作者全名：Yao, Miao; Wang, Kun; Song, Guangming; Hu, Yumeng; Chen, Jiali; Li, Tingting; Liang, Longying; Wu, Jie; Xu, Hongmei; Wang, Libin; Zheng, Yuqiang; Zhang, Xuemei; Yin, Yibing; Yao, Shifei; Wu, Kaifeng
作者地址：[Yao, Miao; Wang, Kun; Song, Guangming; Hu, Yumeng; Chen, Jiali; Li, Tingting; Liang, Longying; Yao, Shifei; Wu, Kaifeng] Zunyi Med Univ, Peoples Hosp Zunyi 1, Dept Lab Med, Affiliated Hosp 3, Zunyi, Guizhou, Peoples R China; [Yao, Miao; Wang, Kun; Song, Guangming; Hu, Yumeng; Chen, Jiali; Li, Tingting; Liang, Longying; Wu, Jie; Yao, Shifei; Wu, Kaifeng] Zunyi Med Univ, Affiliated Hosp 3, Peoples Hosp Zunyi 1, Sci Res Ctr, Zunyi, Guizhou, Peoples R China; [Xu, Hongmei; Wang, Libin; Zheng, Yuqiang; Zhang, Xuemei; Yin, Yibing] Chongqing Med Univ, Sch Lab Med, Key Lab Lab Med Diagnost Designated, Minist Educ, Chongqing, Peoples R China
通信作者：Yao, SF; Wu, KF (通讯作者)，Zunyi Med Univ, Peoples Hosp Zunyi 1, Dept Lab Med, Affiliated Hosp 3, Zunyi, Guizhou, Peoples R China.; Yao, SF; Wu, KF (通讯作者)，Zunyi Med Univ, Affiliated Hosp 3, Peoples Hosp Zunyi 1, Sci Res Ctr, Zunyi, Guizhou, Peoples R China.
来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ESI学科分类：MICROBIOLOGY
WOS号：WOS:001228400300001
JCR分区：Q1
影响因子：4.6
年份：2024
卷号：14
期号：
开始页：
结束页：
文献类型：Article
关键词：Streptococcus pneumoniae; transformation; competence; teichoic acids; ComE; transcriptional regulation
摘要：Competence development is essential for bacterial transformation since it enables bacteria to take up free DNA from the surrounding environment. The regulation of teichoic acid biosynthesis is tightly controlled during pneumococcal competence; however, the mechanism governing this regulation and its impact on transformation remains poorly understood. We demonstrated that a defect in lipoteichoic acid ligase (TacL)-mediated lipoteichoic acids (LTAs) biosynthesis was associated with impaired pneumococcal transformation. Using a fragment of tacL regulatory probe as bait in a DNA pulldown assay, we successfully identified several regulatory proteins, including ComE. Electrophoretic mobility shift assays revealed that phosphomimetic ComE, but not wild-type ComE, exhibited specific binding to the probe. DNase I footprinting assays revealed the specific binding sequences encompassing around 30 base pairs located 31 base pairs upstream from the start codon of tacL. Expression of tacL was found to be upregulated in the Delta comE strain, and the addition of exogenous competence-stimulating peptide repressed the tacL transcription in the wild-type strain but not the Delta comE mutant, indicating that ComE exerted a negative regulatory effect on the transcription of tacL. Mutation in the JH2 region of tacL upstream regulatory sequence led to increased LTAs abundance and displayed higher transformation efficiency. Collectively, our work identified the regulatory mechanisms that control LTAs biosynthesis during competence and thereby unveiled a repression mechanism underlying pneumococcal transformation.
基金机构：Guizhou High-level (BAI) Innovative Talents Project [[2022]042-1]; Graduate Education Innovation Program Project of Zunyi Medical University [ZYK144]; Key Discipline Project of Clinical Laboratory Diagnostics - Guizhou Provincial Health Commission [QianWeijianhan [2021]160]; Shanghai Municipal Health Commission [20215024]; Key Discipline Project of Clinical Laboratory Diagnostics - Zunyi Municipal Health Bureau [2022-1444]
基金资助正文：The author(s) declare financial support was received for the research, authorship, and/or publication of this article. Financial support for this study was provided by the Guizhou High-level (BAI) Innovative Talents Project (QIANKehe Platform & Talents-GCC [2022]042-1), the Graduate Education Innovation Program Project of Zunyi Medical University (ZYK144), the Key Discipline Project of Clinical Laboratory Diagnostics funded by Guizhou Provincial Health Commission (QianWeijianhan [2021]160), Scientific project provided by Shanghai Municipal Health Commission (20215024), and the Key Discipline Project of Clinical Laboratory Diagnostics funded by Zunyi Municipal Health Bureau (2022-1444).
