Integrated analysis of transcriptional changes in major depressive disorder: Insights from blood and anterior cingulate cortex
作者全名：Zhong, Xiaogang; Chen, Xiangyu; Liu, Yiyun; Gui, Siwen; Pu, Juncai; Wang, Dongfang; Tao, Wei; Chen, Yue; Chen, Xiang; Chen, Weiyi; Chen, Xiaopeng; Qiao, Renjie; Tao, Xiangkun; Li, Zhuocan; Xie, Peng
作者地址：[Zhong, Xiaogang; Xie, Peng] Chongqing Med Univ, Coll Basic Med, Chongqing 400016, Peoples R China; [Zhong, Xiaogang; Chen, Xiangyu; Liu, Yiyun; Gui, Siwen; Pu, Juncai; Wang, Dongfang; Tao, Wei; Chen, Yue; Chen, Xiang; Chen, Weiyi; Chen, Xiaopeng; Qiao, Renjie; Tao, Xiangkun; Li, Zhuocan; Xie, Peng] Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Zhong, Xiaogang; Liu, Yiyun; Gui, Siwen; Pu, Juncai; Wang, Dongfang; Tao, Wei; Chen, Yue; Chen, Xiang; Chen, Weiyi; Chen, Xiaopeng; Xie, Peng] Jin Feng Lab, Chongqing 401329, Peoples R China; [Chen, Xiangyu; Pu, Juncai; Chen, Yue; Chen, Xiang; Chen, Weiyi; Chen, Xiaopeng; Qiao, Renjie; Tao, Xiangkun; Li, Zhuocan; Xie, Peng] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing 400016, Peoples R China
通信作者：Xie, P (通讯作者)，Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Affiliated Hosp 1, Chongqing 400016, Peoples R China.
来源：HELIYON
ESI学科分类：
WOS号：WOS:001228770400001
JCR分区：Q1
影响因子：3.4
年份：2024
卷号：10
期号：8
开始页：
结束页：
文献类型：Article
关键词：Major depressive disorder; Blood; Anterior cingulate cortex; Biomarker; Association
摘要：Background: Major depressive disorder (MDD) was involved in widely transcriptional changes in central and peripheral tissues. While, previous studies focused on single tissues, making it difficult to represent systemic molecular changes throughout the body. Thus, there is an urgent need to explore the central and peripheral biomarkers with intrinsic correlation. Methods: We systematically retrieved gene expression profiles of blood and anterior cingulate cortex (ACC). 3 blood datatsets (84 MDD and 88 controls) and 6 ACC datasets (100 MDD and 100 controls) were obtained. Differential expression analysis, RobustRankAggreg (RRA) analysis, functional enrichment analysis, immune associated analysis and protein -protein interaction networks (PPI) were integrated. Furthermore, the key genes were validated in an independent ACC dataset (12 MDD and 15 controls) and a cohort with 120 MDD and 117 controls. Results: Differential expression analysis identified 2211 and 2021 differential expressed genes (DEGs) in blood and ACC, respectively. RRA identified 45 and 25 robust DEGs in blood and ACC based on DEGs, and all of them were closely associated with immune cells. Functional enrichment results showed both the robust DEGs in blood and ACC were enriched in humoral immune response. Furthermore, PPI identified 8 hub DEGs (CD79A, CD79B, CD19, MS4A1, PLP1, CLDN11, MOG, MAG) in blood and ACC. Independent ACC dataset showed the area under the curve (AUC) based on these hub DEGs was 0.77. Meanwhile, these hub DEGs were validated in the serum of MDD patients, and also showed a promising diagnostic power. Conclusions: The biomarker panel based on hub DEGs yield a promising diagnostic efficacy, and all of these hub DEGs were strongly correlated with immunity. Humoral immune response may be the key link between the brain and blood in MDD, and our results may provide further understanding for MDD.
基金机构：National Key Research and Development Program of China [2017YFA0505700]; Natural Science Foundation Project of China [81820108015, 82101596, 82371526]; Joint project of Chongqing Municipal Science and Technology Bureau and Chongqing Health Commission [2023CCXM003]; Postdoctoral Research Foundation of China [2022MD723735, 2022MD723742]; Natural Science Foundation Project of Chongqing [cstc2022ycjh-bgzxm0033]; Young Elite Scientists Sponsorship Program by CAST [2021QNRC001]; Chongqing Postdoctoral Science Foundation [2022NSCQ-BHX1283]
基金资助正文：This work was supported by the National Key Research and Development Program of China (2017YFA0505700) , Natural Science Foundation Project of China (81820108015, 82101596, 82371526) , the Joint project of Chongqing Municipal Science and Technology Bureau and Chongqing Health Commission (2023CCXM003) , the Postdoctoral Research Foundation of China (2022MD723735, 2022MD723742) , the Natural Science Foundation Project of Chongqing (cstc2022ycjh-bgzxm0033) , Young Elite Scientists Sponsorship Program by CAST (2021QNRC001) , the Chongqing Postdoctoral Science Foundation (2022NSCQ-BHX1283) .
